关键信息
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基因名
SIRT3
- 应用
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别名
SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3
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种属
Rat
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
C6ZII9
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表达区间
Pro127~Glu303
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分子量
22kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SIRT3, a member of the sirtuin family of proteins, has garnered significant attention in recent years due to its critical role in regulating metabolic processes and cellular stress responses. As a mitochondrial deacetylase, SIRT3 is involved in the desacetylation of key metabolic enzymes, thereby influencing energy expenditure, oxidative stress, and overall mitochondrial function. Research has illuminated its implications in age-related diseases, including obesity, diabetes, and neurodegenerative disorders, making it a potential target for therapeutic interventions. Studies have demonstrated that SIRT3 activation can enhance mitochondrial function, improve insulin sensitivity, and reduce inflammation, which are crucial in maintaining cellular homeostasis. Furthermore, the exploration of SIRT3 recombinant proteins has provided a valuable tool for understanding its enzymatic mechanisms and identifying potential substrates. These insights could lead to the development of SIRT3-modulating drugs, with the aim of improving health span and combating age-related pathologies. Overall, SIRT3's role in metabolism and cellular health places it at the forefront of current biomedical research, reflecting the importance of mitochondrial regulation in disease prevention and treatment.
