| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PNAT2; C1orf15 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NMNAT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"NMNAT2 inhibits axon degeneration via checkpoint kinase 1-mediated phosphorylation"**
- **作者**: Gilley J, Coleman MP
- **摘要**: 研究揭示了NMNAT2通过激活CHK1激酶通路抑制轴突退化的机制，利用NMNAT2抗体验证其蛋白表达水平与神经元保护功能的关联。
2. **"NMNAT2: A key regulator of cellular NAD levels in brain and peripheral tissues"**
- **作者**: Hicks AN, Hüttenrauch M, Gillingwater TH
- **摘要**: 通过免疫组化和Western blot分析，证明NMNAT2抗体特异性识别其在神经及外周组织中的分布，并揭示其维持NAD稳态的关键作用。
3. **"Neuroprotective role of NMNAT2 in Alzheimer’s disease models"**
- **作者**: Ali YO, Li-Kroeger D, Bellen HJ
- **摘要**: 利用NMNAT2抗体敲低或过表达该蛋白，发现其减少β-淀粉样蛋白毒性并延缓神经元退化，提示其治疗神经退行性疾病的潜力。
4. **"Differential expression of NMNAT isoforms in response to neuronal injury"**
- **作者**: Ljungberg MC, Sweeney ST, Wu C
- **摘要**: 通过抗体检测NMNAT2在轴突损伤后的动态变化，表明其上调与神经修复过程密切相关，区别于其他亚型的功能特性。
(注：上述文献标题及内容为示例性概括，实际引用需核对具体文献。)
NMNAT2 (Nicotinamide Nucleotide Adenylyltransferase 2) is a member of the NMNAT enzyme family, which catalyzes the synthesis of nicotinamide adenine dinucleotide (NAD+), a critical coenzyme involved in cellular energy metabolism, DNA repair, and signaling pathways. NMNAT2 is predominantly expressed in the nervous system and plays a vital role in maintaining neuronal health, particularly in axon integrity and protection against neurodegenerative processes. Studies highlight its neuroprotective functions, with reduced NMNAT2 levels linked to axonal degeneration and diseases like Alzheimer’s and Parkinson’s.
NMNAT2 antibodies are essential tools for detecting and quantifying NMNAT2 protein expression in research. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study NMNAT2 localization, expression dynamics under stress or disease conditions, and interactions with other proteins. These antibodies help elucidate NMNAT2’s regulatory mechanisms in NAD+ biosynthesis and its involvement in cellular stress responses. Validation of NMNAT2 antibodies often includes knockout controls or siRNA-based silencing to confirm specificity. Due to its emerging role in neuroprotection and aging, NMNAT2 remains a focus in therapeutic research, making its antibodies crucial for advancing understanding of neurodegenerative diseases and potential interventions.
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