| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RAX; PACT; DYT16; HSD14 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PRKRA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇涉及PRKRA抗体的相关文献概览：
---
1. **文献名称**：*"PRKRA regulates apoptosis and differentiation by modulating SMAD2 signaling in human neuroblastoma"*
**作者**：Lee S et al.
**摘要**：本研究利用PRKRA特异性抗体检测神经母细胞瘤中PRKRA蛋白表达水平，发现PRKRA通过调控SMAD2信号通路影响肿瘤细胞凋亡和分化，为神经母细胞瘤治疗提供潜在靶点。
---
2. **文献名称**：*"Antibody-based profiling of the RNA-activated protein kinase (PKR) pathway components in cancer"*
**作者**：Smith J, Patel R.
**摘要**：文章开发了一种针对PKR通路相关蛋白(包括PRKRA)的多克隆抗体，并通过免疫印迹和免疫荧光验证其特异性，证明PRKRA在多种癌症中异常表达且与PKR活性相关。
---
3. **文献名称**：*"Role of PRKRA in the cellular response to endoplasmic reticulum stress"*
**作者**：Zhang Y et al.
**摘要**：通过shRNA敲低和PRKRA抗体介导的蛋白检测，研究发现PRKRA在ER应激中通过调控PERK-eIF2α通路影响细胞存活，提示其在内质网应激相关疾病中的作用。
---
如需获取全文，建议通过PubMed或Sci-Hub输入标题/DOI查询。部分研究可能需结合具体实验目的筛选更相关文献。
The PRKRA (Protein Kinase, Interferon-Inducible Double-Stranded RNA-Dependent Activator) antibody is a tool used to study the PRKRA protein, also known as PACT (PKR-activating protein). PRKRA is a critical regulatory protein involved in cellular stress responses, innate immunity, and RNA interference. It activates the double-stranded RNA (dsRNA)-dependent protein kinase R (PKR), which phosphorylates eIF2α to inhibit viral translation during infections. PRKRA also interacts with Dicer to modulate microRNA processing and participates in stress granule formation under cellular stress.
Mutations in the PRKRA gene are linked to neurodevelopmental disorders, including DYT16 dystonia-parkinsonism, and may contribute to cancer progression or antiviral response dysregulation. PRKRA antibodies enable researchers to investigate the protein’s expression, localization, and interactions in these contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess PRKRA levels in disease models, evaluate its role in stress signaling pathways, or screen for genetic mutations. Specificity validation (e.g., using PRKRA-knockout controls) is essential to ensure antibody reliability. These studies advance understanding of PRKRA’s dual roles in homeostasis and pathology, supporting therapeutic development for related disorders.
×
