首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠补体因子I(CFI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CFI含量。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于科研领域对补体系统相关机制的研究，如探究炎症性疾病、免疫调控或感染过程中CFI的表达变化。可用于细胞模型、动物实验或生物标志物筛选等基础研究场景，助力补体系统功能解析及免疫相关疾病机制的深入探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cfi ELISA Kit; IfComplement factor I ELISA Kit; EC 3.4.21.45 ELISA Kit; C3B/C4B inactivator) [Cleaved into: Complement factor I heavy chain; Complement factor I light chain] ELISA Kit
缩写：CFI
Uniprot No.：Q61129
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CFI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 97 | |||||
| Range % | 93-100 | ||||||
| 1:2000 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:4000 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:8000 | Average % | 87 | |||||
| Range % | 85-90 | ||||||
回收率：
| The recovery of mouse CFI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 98-106 | |||||
| EDTA plasma (n=4) | 85 | 81-89 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected502.2302.3012.2662.161252.0201.9872.0041.89912.51.6011.7001.6511.5466.251.0851.1251.1051.0003.120.5960.5680.5820.4771.560.3570.3610.3590.2540.780.2050.2120.2090.10400.1020.1080.105 | |||||||
货期：3-5 working days
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