| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | THO3; hTREX45 |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human THOC3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于THOC3抗体的3篇文献摘要示例(文献信息为虚构示例，仅供格式参考)：
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1. **文献名称**: *THOC3 regulates ovarian cancer progression via mRNA export pathways*
**作者**: Zhang L, et al.
**摘要**: 本研究利用特异性THOC3抗体，通过免疫沉淀和Western blot验证其在卵巢癌细胞中的高表达，发现THOC3通过调控致癌基因mRNA的核质转运促进肿瘤侵袭。
2. **文献名称**: *THOC3 antibody validation in colorectal cancer models*
**作者**: Kim S, et al.
**摘要**: 研究开发了一种高灵敏度的兔源THOC3多克隆抗体，用于免疫组化分析结直肠癌组织，证实THOC3表达与患者预后不良显著相关。
3. **文献名称**: *THOC3 deficiency impairs mouse embryonic development*
**作者**: Müller R, et al.
**摘要**: 通过THOC3抗体检测敲除小鼠模型，发现THOC3缺失导致胚胎致死，并破坏THO复合体稳定性，影响RNA加工通路。
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注：实际文献需通过PubMed或Google Scholar检索关键词“THOC3 antibody”或“THOC3 function”，建议查阅《Oncogene》《Cell Reports》等期刊近期研究。
The THOC3 antibody is a crucial tool for studying the THO complex subunit 3 (THOC3), a component of the evolutionarily conserved THO complex involved in mRNA processing and export. The THO complex, comprising THOC1-THOC7 proteins, interacts with other mRNA export factors (e.g., ALYREF, UAP56) to form the Transcription-Export (TREX) complex, which couples transcription elongation with nuclear mRNA export. THOC3. a 33 kDa protein, plays a structural role in stabilizing the THO complex and ensuring proper mRNA splicing and transport. Dysregulation of THOC3 is linked to developmental defects, neurodevelopmental disorders, and cancers, as it influences the expression of genes critical for cell proliferation and differentiation.
THOC3 antibodies are widely used to investigate its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help elucidate THOC3's role in maintaining mRNA stability, preventing R-loop formation, and mediating cellular stress responses. Commercial THOC3 antibodies are typically raised against specific epitopes (e.g., recombinant human THOC3 fragments) and validated for specificity across human, mouse, and rat models. Research utilizing these antibodies has highlighted THOC3's importance in embryogenesis, neuronal function, and cancer progression, making it a biomarker of interest in both basic and clinical studies.
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