Rabbit Polyclonal RAD23A Antibody

The RAD23A antibody is a crucial tool for studying the RAD23 homolog A protein, a key player in DNA repair and ubiquitin-mediated proteolysis. RAD23A belongs to the RAD23 family, which includes RAD23B, both sharing structural domains: an N-terminal ubiquitin-like (UBL) domain and two ubiquitin-associated (UBA) domains. These domains enable interactions with the 26S proteasome and polyubiquitinated substrates, linking RAD23A to the nucleotide excision repair (NER) pathway and protein degradation processes. In NER, RAD23A partners with XPC to recognize and repair UV-induced DNA lesions or chemical adducts, ensuring genomic stability. Additionally, RAD23A assists in shuttling ubiquitinated proteins to the proteasome, balancing protein homeostasis. Dysregulation of RAD23A is implicated in cancer progression and neurodegenerative diseases due to defective DNA repair or protein aggregation. Researchers use RAD23A antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate its expression, localization, and interactions in cellular models. These antibodies (often raised in rabbits or mice) are validated for specificity, with cross-reactivity checks against RAD23B. Studies employing RAD23A antibodies contribute to understanding DNA damage responses, proteostasis networks, and therapeutic targets for diseases linked to genomic instability or protein misfolding.