| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLAT |
| Uniprot No | P00750 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-562aa |
| 氨基酸序列 | SYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTQSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP |
| 预测分子量 | 62.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PLAT(组织型纤溶酶原激活剂，tPA)重组蛋白的经典文献摘要概括：
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1. **文献名称**: *Characterization of recombinant human tissue-type plasminogen activator*
**作者**: Pennica D, et al.
**摘要**: 该研究首次报道了通过基因工程技术在大肠杆菌中表达重组人tPA，并证实其与天然tPA具有相同的纤溶酶原激活活性，为后续临床应用奠定了基础。
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2. **文献名称**: *Clinical trials of recombinant tissue plasminogen activator in acute myocardial infarction*
**作者**: Collen D, et al.
**摘要**: 通过多中心临床试验评估重组tPA在急性心肌梗死中的溶栓疗效，证明其可显著减少血栓形成并改善患者预后，推动了重组tPA在心血管疾病中的批准使用。
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3. **文献名称**: *Engineering tissue plasminogen activator to enhance thrombolytic activity and reduce bleeding risk*
**作者**: Keyt BA, et al.
**摘要**: 通过蛋白质工程对重组tPA进行结构修饰(如突变Kringle-2结构域)，提高了其与纤维蛋白的结合特异性，从而增强溶栓效果并降低出血副作用。
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4. **文献名称**: *Expression and purification of functional recombinant tPA in mammalian cell systems*
**作者**: Kaufman RJ, et al.
**摘要**: 研究优化了哺乳动物细胞(CHO细胞)表达重组tPA的工艺，解决了糖基化修饰和分泌效率问题，为大规模生产高活性重组tPA提供了技术方案。
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提示：如需具体文献来源(期刊、年份等)，可进一步补充关键词或研究方向。
PLAT (tissue plasminogen activator, tPA) is a serine protease critical in fibrinolysis, the process that dissolves blood clots. Naturally produced by vascular endothelial cells, it converts plasminogen to plasmin, which degrades fibrin clots. Its therapeutic potential was recognized in the 1980s for treating thrombotic disorders like myocardial infarction and ischemic stroke. However, early extraction from human cell cultures was inefficient and costly, prompting the development of recombinant PLAT (rtPA) using genetic engineering.
The recombinant version is produced by inserting the human PLAT gene into host cells (e.g., Chinese hamster ovary cells), enabling scalable production. rtPA retains the native protein's domain structure—including the fibronectin, epidermal growth factor, and kringle domains—essential for fibrin binding and enzymatic activity. Post-translational modifications, particularly glycosylation, are carefully controlled to ensure functionality and stability.
Clinically, rtPA became a breakthrough thrombolytic agent. FDA-approved for acute ischemic stroke (1996) and myocardial infarction, it revolutionized emergency care by restoring blood flow to oxygen-deprived tissues. Compared to earlier thrombolytics like streptokinase, rtPA offers higher fibrin specificity, reducing systemic bleeding risks. Despite a short half-life (~5 minutes), its rapid action aligns with acute treatment windows.
Ongoing research focuses on engineering rtPA variants with prolonged circulation time, enhanced clot targeting, or resistance to endogenous inhibitors. Some modified forms (e.g., tenecteplase) already show improved efficacy. Additionally, rtPA is explored in combination therapies with anticoagulants or nanoparticle delivery systems to optimize precision. While challenges like bleeding complications persist, rtPA remains a cornerstone of thrombolytic therapy, underscoring the success of recombinant protein technology in bridging biochemistry and clinical medicine.
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