| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BDP; PTMP; PP2Cm; MSUDMV; PP2Ckappa; UG0882E07 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PPM1K |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPM1K抗体的3篇示例文献(内容基于公开研究领域知识整理，具体文献需通过学术数据库验证)：
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1. **文献名称**：*"PPM1K regulates hepatic insulin sensitivity through the PP2C-dependent dephosphorylation of AMPK"*
**作者**：Zhang Y, et al.
**摘要**：研究揭示了PPM1K通过去磷酸化AMPK调控肝脏胰岛素信号通路的作用，利用特异性PPM1K抗体证实其在肝细胞中的表达水平与代谢综合征的相关性。
2. **文献名称**：*"Role of PPM1K in neuronal apoptosis and its potential as a therapeutic target in neurodegenerative diseases"*
**作者**：Wang L, et al.
**摘要**：通过免疫印迹和免疫组化实验(使用PPM1K抗体)，发现PPM1K在神经元凋亡中的关键作用，并探讨其作为阿尔茨海默病等疾病的治疗靶点潜力。
3. **文献名称**：*"PPM1K deficiency exacerbates cardiac ischemia-reperfusion injury via modulating mitochondrial function"*
**作者**：Liu X, et al.
**摘要**：研究利用PPM1K敲除小鼠模型及抗体检测技术，证明PPM1K缺失通过线粒体功能障碍加重心脏缺血再灌注损伤，提示其保护性机制。
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**备注**：以上文献信息为示例性质，实际引用请通过PubMed、Google Scholar或Web of Science等平台检索真实发表的论文(关键词：PPM1K antibody, phosphatase, PPM1K function)。如需具体文献DOI或年份，建议补充研究背景后进一步查询。
**Background of PPM1K Antibody**
PPM1K (Protein Phosphatase Mg²⁺/Mn²⁺-Dependent 1K), also known as PP2Cκ or POPCK, is a member of the PPM family of serine/threonine phosphatases that require Mg²⁺ or Mn²⁺ for catalytic activity. It plays a critical role in regulating mitochondrial metabolism, particularly in the catabolism of branched-chain amino acids (BCAAs) by dephosphorylating and activating the branched-chain α-keto acid dehydrogenase (BCKDH) complex. Dysregulation of PPM1K is linked to metabolic disorders, including diabetes, cardiovascular diseases, and neurological conditions.
Antibodies targeting PPM1K are essential tools for studying its expression, localization, and function in cellular pathways. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate PPM1K’s role in stress responses, energy homeostasis, and disease mechanisms. For instance, studies have utilized PPM1K antibodies to explore its interaction with AMP-activated protein kinase (AMPK) and mTOR signaling pathways, which are central to metabolic regulation.
Commercial PPM1K antibodies are typically developed in rabbits or mice, using immunogenic peptides or recombinant protein fragments. Validation often includes knockout cell lines or tissues to confirm specificity. Research-grade antibodies help uncover PPM1K’s therapeutic potential, particularly in modulating BCAA metabolism and mitigating metabolic syndrome-related pathologies.
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