| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Bcl-2; BCL2 |
| Entrez GeneID | 596 |
| clone | 8E12 |
| WB Predicted band size | 26kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于HSD3B2抗体的3篇参考文献的简要总结(内容基于模拟文献，仅供参考)：
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1. **文献名称**：*Immunohistochemical analysis of HSD3B2 in congenital adrenal hyperplasia*
**作者**：Smith A, et al.
**摘要**：本研究利用HSD3B2特异性抗体对先天性肾上腺增生症(CAH)患者的肾上腺组织进行免疫组化分析，发现HSD3B2蛋白表达显著降低，与基因突变导致的3β-羟基类固醇脱氢酶Ⅱ型功能缺陷相关。
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2. **文献名称**：*Development of a monoclonal antibody against human HSD3B2 for enzymatic activity detection*
**作者**：Chen L, et al.
**摘要**：作者开发了一种针对HSD3B2的单克隆抗体，并通过Western blot和ELISA验证其特异性。该抗体成功用于检测细胞模型中HSD3B2的酶活性水平，为相关代谢疾病的诊断提供了新工具。
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3. **文献名称**：*HSD3B2 mutations and protein expression in steroidogenic disorders*
**作者**：García-Rúa R, et al.
**摘要**：研究通过HSD3B2抗体分析突变患者的成纤维细胞，发现部分错义突变虽不影响mRNA水平，但导致蛋白稳定性下降，揭示了抗体在功能研究中对突变蛋白定量的重要性。
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4. **文献名称**：*Autoantibodies to HSD3B2 in autoimmune adrenal insufficiency*
**作者**：Tanaka K, et al.
**摘要**：首次报道在自身免疫性肾上腺功能不全患者血清中检测到抗HSD3B2的自身抗体，提示该抗体可能参与疾病发生，为自身免疫性内分泌疾病的机制研究提供了新方向。
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(注：以上文献为示例，实际引用需以真实发表的论文为准。)
The HSD3B2 antibody targets the enzyme 3β-hydroxysteroid dehydrogenase type 2 (HSD3B2), a critical protein in steroid hormone biosynthesis. HSD3B2 is primarily expressed in the adrenal glands and gonads, where it catalyzes the conversion of Δ⁵-steroids (e.g., pregnenolone, dehydroepiandrosterone) into Δ⁴-steroids (e.g., progesterone, androstenedione), a key step in producing cortisol, aldosterone, and sex hormones. Mutations in the HSD3B2 gene are linked to congenital adrenal hyperplasia (CAH), a disorder characterized by impaired cortisol synthesis, adrenal insufficiency, and androgen excess. Antibodies against HSD3B2 are essential tools for studying its expression, localization, and function in both normal and pathological tissues.
In research, HSD3B2 antibodies are used in immunohistochemistry, Western blotting, and immunofluorescence to investigate steroidogenic pathways, adrenal disorders, or reproductive endocrinology. They help differentiate HSD3B2 from its isoform HSD3B1. which is expressed in peripheral tissues and plays a distinct role in hormone metabolism. Clinically, these antibodies aid in diagnosing CAH subtypes caused by HSD3B2 deficiency, guiding genetic counseling and therapeutic strategies. Their specificity also supports studies on hormone-dependent cancers, such as prostate or breast cancer, where altered steroidogenesis may influence disease progression. Overall, HSD3B2 antibodies are vital for advancing understanding of steroid biology and related pathologies.
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