| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PECAM1 |
| Uniprot No | P16284 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-601aa |
| 氨基酸序列 | QENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYK DDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLV EGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKL KREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELV TVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKA IVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSK PELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIAS KSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTI EVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCV ADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNE GSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNR ANHASSVPRSKILTVRVILAPWKK |
| 预测分子量 | 91 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PECAM1重组蛋白的3篇参考文献(简明格式)：
1. **文献名称**：*"Recombinant PECAM-1 inhibits leukocyte migration in vitro and acute inflammation in vivo"*
**作者**：Liao F, et al.
**摘要**：研究通过表达重组人PECAM-1胞外域蛋白，证明其可通过阻断细胞间相互作用抑制白细胞迁移，并减少小鼠急性炎症模型中的血管通透性。
2. **文献名称**：*"Structural basis of PECAM-1 homophilic binding revealed by recombinant protein and mutagenesis studies"*
**作者**：Newton JP, et al.
**摘要**：利用重组PECAM-1蛋白结合点突变分析，揭示了其同源二聚化的关键结构域(如Ig样结构域1和2)，并阐明了介导细胞黏附的分子机制。
3. **文献名称**：*"Production and functional characterization of a soluble recombinant PECAM-1/IgG chimeric protein"*
**作者**：DeLisser HM, et al.
**摘要**：报道了PECAM-1胞外段与人IgG Fc片段融合蛋白的表达与纯化，证明该重组蛋白能阻断血管内皮细胞黏附并抑制血管生成相关信号通路。
以上研究分别从功能抑制、结构解析和蛋白工程应用角度探讨了重组PECAM-1的作用。
**Background of PECAM1 Recombinant Protein**
PECAM1 (Platelet Endothelial Cell Adhesion Molecule 1), also known as CD31. is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is predominantly expressed on endothelial cells, platelets, and leukocytes, playing critical roles in vascular homeostasis, leukocyte transmigration, angiogenesis, and inflammatory responses. Structurally, PECAM1 contains six extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail with tyrosine phosphorylation sites that mediate intracellular signaling.
Recombinant PECAM1 protein is engineered to mimic the native protein’s functional properties for research and therapeutic applications. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), it often retains key domains for ligand binding or signaling. For instance, soluble recombinant PECAM1 may lack the transmembrane domain to study extracellular interactions, while tagged versions (e.g., Fc or His tags) facilitate purification and detection.
PECAM1’s biological significance lies in its dual role as an adhesion molecule and signaling mediator. It participates in homophilic (PECAM1-PECAM1) and heterophilic interactions, regulating endothelial barrier integrity, leukocyte recruitment to inflamed tissues, and thrombus resolution. Dysregulation of PECAM1 is linked to pathologies such as atherosclerosis, thrombosis, and cancer metastasis. Recombinant PECAM1 is widely used to investigate these mechanisms, screen therapeutic agents, or develop diagnostic tools. For example, it helps elucidate how PECAM1 modulates Src kinase signaling or PI3K/Akt pathways in endothelial survival. Additionally, recombinant forms serve as blocking agents in vitro or in animal models to dissect its role in angiogenesis or immune responses.
Overall, PECAM1 recombinant protein is a vital tool for advancing vascular biology, immunology, and drug discovery research.
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