| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IPS; INO1; INOS; IPS 1; IPS-1 |
| WB Predicted band size | 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ISYNA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于 **ISYNA1抗体** 的模拟参考文献(根据学术文献常见内容虚构，供参考)：
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1. **文献名称**: *"Development and validation of a polyclonal antibody against human ISYNA1 for inositol biosynthesis studies"*
**作者**: Berger, H., et al.
**摘要**: 本研究开发并验证了一种针对人源ISYNA1蛋白的多克隆抗体，通过免疫印迹(Western blot)和免疫荧光技术证实其特异性。抗体成功应用于检测多种细胞系中ISYNA1的表达水平，并揭示其在亚细胞定位中的分布特征，为研究肌醇代谢通路提供工具支持。
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2. **文献名称**: *"ISYNA1 knockdown impairs lipid metabolism: Insights from antibody-based protein quantification in liver tissues"*
**作者**: Smith, J., et al.
**摘要**: 通过RNA干扰技术敲低ISYNA1基因，结合商业化ISYNA1抗体的免疫组化分析，发现ISYNA1缺失导致小鼠肝脏组织肌醇合成减少，并伴随甘油三酯异常积累。研究强调了ISYNA1在脂代谢中的关键作用及抗体在组织水平检测中的应用价值。
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3. **文献名称**: *"A novel monoclonal antibody targeting ISYNA1 reveals elevated expression in breast cancer progression"*
**作者**: Lee, M., et al.
**摘要**: 本研究报道了一种高特异性单克隆抗体，可识别ISYNA1的C端表位。通过乳腺癌临床样本分析，发现ISYNA1在肿瘤组织中的表达显著高于正常组织，且与患者生存率负相关，提示其作为潜在生物标志物的可能性。
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**备注**：以上文献为示例性内容，实际研究中需通过PubMed或Google Scholar检索真实文献(关键词：ISYNA1 antibody, inositol synthase, myo-inositol biosynthesis)。
The ISYNA1 antibody is a research tool designed to detect inositol-3-phosphate synthase 1 (ISYNA1), an enzyme critical for synthesizing myo-inositol, a key molecule in cellular signaling and metabolic processes. ISYNA1 catalyzes the conversion of glucose-6-phosphate to inositol-3-phosphate, the rate-limiting step in myo-inositol production. Myo-inositol serves as a precursor for phosphatidylinositol phosphates, which regulate membrane trafficking, ion channel activity, and growth factor signaling. Dysregulation of ISYNA1 has been implicated in neurological disorders, cancer, and metabolic syndromes, making it a target for studying disease mechanisms.
ISYNA1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression and localization in tissues or cultured cells. These antibodies aid in exploring ISYNA1's role in cellular responses to stress, insulin signaling, and neural development. Commercial ISYNA1 antibodies are typically raised in rabbits or mice, validated for specificity using knockout (KO) controls or siRNA knockdown. Researchers also utilize them to investigate correlations between ISYNA1 expression levels and pathological conditions, such as tumor progression or neurodevelopmental defects. Proper validation remains essential to ensure accurate interpretation of experimental results in diverse biological contexts.
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