| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HLP4 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human HPCAL4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HPCAL4抗体的模拟参考文献示例(注：文献为虚构，仅作格式参考)：
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1. **"Characterization of HPCAL4 Antibody for Detection of Calcium-Binding Proteins in Neuronal Tissues"**
*Authors: Zhang L, et al.*
摘要：本研究开发了一种针对HPCAL4蛋白的多克隆抗体，并通过Western blot和免疫组化验证其特异性。实验表明，该抗体能特异性识别脑组织中的HPCAL4.并揭示其在海马体神经元中的高表达，提示其可能与突触可塑性相关。
2. **"HPCAL4 Expression in Glioblastoma: A Prognostic Marker Study Using Immunohistochemistry"**
*Authors: Patel R, et al.*
摘要：通过商业化HPCAL4抗体(克隆号：ABX-204)分析胶质母细胞瘤患者的组织样本。研究发现，HPCAL4高表达与患者生存期缩短显著相关，提示其作为肿瘤恶性程度的潜在生物标志物。
3. **"Subcellular Localization of HPCAL4 in Retinal Photoreceptors: An Antibody-Based Imaging Approach"**
*Authors: Müller S, et al.*
摘要：利用免疫荧光技术结合HPCAL4抗体，首次报道了该蛋白在视网膜感光细胞中的亚细胞定位。结果显示HPCAL4主要富集于纤毛基部，可能参与光信号转导中的钙离子调控。
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**备注**：若需真实文献，建议通过PubMed或Google Scholar以“HPCAL4 antibody”为关键词检索，并筛选涉及抗体验证、蛋白功能或疾病关联的研究。
The HPCAL4 antibody targets the Hippocalcin-like 4 protein (HPCAL4), a member of the neuronal calcium sensor (NCS) family, which plays roles in calcium-mediated signaling pathways. HPCAL4. also known as VSNL1-like protein, is encoded by the HPCAL4 gene located on human chromosome 1q25.3. It contains EF-hand calcium-binding domains and is predominantly expressed in neuronal tissues, though its expression has also been detected in non-neuronal cells. HPCAL4 is implicated in regulating cellular processes such as synaptic plasticity, apoptosis, and intracellular trafficking by modulating calcium-dependent interactions with target proteins.
Antibodies against HPCAL4 are utilized in research to investigate its expression patterns, subcellular localization, and functional roles in both physiological and pathological contexts. For example, studies have explored its potential involvement in neurodegenerative diseases, cancer progression (e.g., prostate cancer), and neurodevelopmental disorders. Western blotting, immunohistochemistry, and immunofluorescence are common applications. However, HPCAL4's precise mechanisms remain less characterized compared to other NCS proteins like hippocalcin or neurocalcin. Commercial antibodies are typically validated for specificity via knockout controls or siRNA knockdown to ensure accurate detection. Ongoing research aims to clarify its interaction networks and therapeutic relevance in calcium-related pathologies.
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