首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠冷诱导RNA结合蛋白(CIRBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CIRBP含量。CIRBP（冷诱导RNA结合蛋白）是一种进化上保守的RNA结合蛋白，包含RNA识别结构域（RRM）和RGG结构域。它在多种应激条件下表达变化，调控转录、转录后和翻译过程。CIRBP在细胞应激反应、基因毒性应激、炎症、神经可塑性、干细胞特性和癌症发生发展中发挥重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于体外检测小鼠模型中CIRBP的动态表达变化，可广泛应用于低温应激模型构建、代谢性疾病机制研究、炎症相关信号通路探索等科研领域，为研究CIRBP在生理调节或病理状态下的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cirbp ELISA kit; CirpCold-inducible RNA-binding protein ELISA kit; A18 hnRNP ELISA kit; Glycine-rich RNA-binding protein CIRP ELISA kit
缩写：CIRBP
Uniprot No.：P60824
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIRBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 96-107 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of mouse CIRBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 93-102 | |||||
| EDTA plasma (n=4) | 103 | 92-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.4902.5672.5292.3975002.0212.1232.0721.9402501.4151.3411.3781.2461250.7910.7820.7870.65562.50.4690.4730.4710.33931.250.2890.2710.2800.14815.60.2100.2140.2120.08000.1310.1320.132 | |||||||
货期：3-5 working days
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