首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肌酸激酶同工酶MM(CK-MM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CK-MM含量。CK-MM即肌酸激酶同工酶，主要存在于骨骼肌和心肌中，在临床上是诊断急性心肌梗死等疾病的重要指标。研究机制方面，通过检测血液中CK-MM的含量变化，可反映心肌或骨骼肌细胞损伤情况，在心肌梗死时其水平会快速升高。试剂盒检测范围为0.156 mU/mL-10 mU/mL，适用于基础科研中骨骼肌损伤模型评估、运动生理代谢研究、肌肉相关疾病机制探索等场景，例如通过监测血清CK-MM浓度变化研究肌肉再生能力或病理损伤程度。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CK-MM
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.156 mU/mL-10 mU/mL
灵敏度：0.039 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 98-108 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-99 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected102.4202.5662.4932.34351.7081.8551.7821.6322.50.9701.0020.9860.8361.250.5560.6010.5790.4290.6250.3590.3690.3640.2140.3120.2700.2780.2740.1240.1560.1970.1940.1960.04600.1550.1450.150 | ||||||
货期：3-5 working days
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