| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | P78; MCRS2; MSP58; INO80Q; ICP22BP |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MCRS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MCRS1抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*MCRS1 Antibody Characterization in Mitotic Regulation*
**作者**：Smith, J. et al.
**摘要**：该研究开发了一种特异性单克隆MCRS1抗体，验证其在免疫荧光(IF)和Western blot中的应用，揭示了MCRS1在调控有丝分裂纺锤体组装中的关键作用。
2. **文献名称**：*Development of a Polyclonal MCRS1 Antibody for Chromatin Interaction Studies*
**作者**：Zhang, L. et al.
**摘要**：报道了一种兔源多克隆抗体的制备方法，通过染色质免疫沉淀(ChIP)实验证实MCRS1与染色质重塑复合体相互作用，影响基因转录调控。
3. **文献名称**：*MCRS1 Overexpression in Hepatocellular Carcinoma Detected by Immunohistochemistry*
**作者**：Lee, H. et al.
**摘要**：利用MCRS1抗体进行免疫组化(IHC)分析，发现肝癌组织中MCRS1蛋白表达显著上调，提示其作为潜在肿瘤标志物的可能性。
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**备注**：以上文献为示例，实际引用时需根据具体研究内容核实来源及细节。建议通过PubMed或Web of Science以关键词“MCRS1 antibody”或“MCRS1 immunodetection”检索最新文献。
The MCRS1 antibody targets the microspherule protein 1 (MCRS1), a multifunctional protein implicated in diverse cellular processes, including cell cycle regulation, RNA processing, and chromatin remodeling. MCRS1 interacts with key partners such as the Aurora A kinase (AURKA) and the tumor suppressor INI1/hSNF5. influencing mitotic progression, centrosome duplication, and genomic stability. Structurally, MCRS1 contains coiled-coil and SEF (similar to elongation factor) domains, enabling interactions with multiple molecular complexes.
MCRS1 is overexpressed in cancers like hepatocellular carcinoma and glioblastoma, correlating with poor prognosis due to its roles in promoting proliferation, metastasis, and apoptosis resistance. Antibodies against MCRS1 are widely used in techniques like Western blotting, immunofluorescence, and ChIP to study its localization, expression dynamics, and interactions. These antibodies typically recognize epitopes within conserved regions, such as the N-terminal domain, and help identify post-translational modifications like phosphorylation that regulate MCRS1 activity.
Commercial MCRS1 antibodies (e.g., from Sigma-Aldrich, Abcam) are often raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation includes detecting the ~75 kDa endogenous protein in cell lysates and confirming specificity via knockdown controls. Research utilizing these antibodies has advanced understanding of MCRS1's oncogenic mechanisms and its potential as a therapeutic target.
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