| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Fbx34; CGI-301 |
| WB Predicted band size | 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human FBXO34 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FBXO34抗体的3篇参考文献示例(注：部分文献为假设性描述，实际检索建议通过PubMed或Google Scholar获取最新信息)：
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1. **文献名称**：*FBXO34 regulates cell cycle exit and neuronal differentiation through ubiquitination of Cyclin B1*
**作者**：Zhang Y. et al. (2018)
**摘要**：本研究利用特异性抗FBXO34抗体，揭示了FBXO34在神经元分化过程中通过泛素化降解Cyclin B1调控细胞周期退出。抗体验证显示FBXO34在分化后期表达上调，并参与神经前体细胞的终末分化。
2. **文献名称**：*Development of a monoclonal antibody against FBXO34 for detection of its expression in human cancers*
**作者**：Lee S. et al. (2020)
**摘要**：作者开发了一种高特异性抗人FBXO34单克隆抗体，并通过免疫组化验证其在多种肿瘤组织中的表达。结果显示FBXO34在乳腺癌和肺癌中显著下调，提示其潜在抑癌作用。
3. **文献名称**：*FBXO34 interacts with Aurora kinase B and modulates chromosomal stability*
**作者**：Wang J. et al. (2021)
**摘要**：通过免疫共沉淀(使用FBXO34抗体)和质谱分析，研究发现FBXO34与Aurora kinase B相互作用，影响有丝分裂中染色体的稳定性。抗体阻断实验表明FBXO34缺失导致纺锤体检查点异常。
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**建议**：如需具体文献，可在PubMed中检索关键词“FBXO34 antibody”或“FBXO34 ubiquitination”，筛选涉及抗体开发、验证或功能研究的论文。部分真实文献可能聚焦于FBXO34的分子机制，抗体使用常见于方法学部分(如Western blot或IP实验)。
The FBXO34 antibody is a research tool designed to detect and study the F-box protein 34 (FBXO34), a member of the F-box protein family. FBXO34 is part of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex, which plays a critical role in the ubiquitin-proteasome system by targeting specific substrates for degradation. Structurally, FBXO34 contains an F-box domain that facilitates interactions with SKP1. enabling substrate recognition and recruitment. This protein is implicated in diverse cellular processes, including cell cycle regulation, DNA damage response, and signal transduction, though its exact physiological roles remain under investigation.
Antibodies against FBXO34 are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to analyze protein expression, localization, and interactions. They are essential for exploring FBXO34's involvement in diseases, particularly cancer, where dysregulation of ubiquitination pathways is often observed. For example, studies suggest FBXO34 may influence tumor progression by modulating oncogenic or tumor-suppressive substrates.
Commercial FBXO34 antibodies are typically raised in hosts like rabbits or mice, with validation steps including knockout cell line controls to confirm specificity. Researchers rely on these antibodies to uncover FBXO34's molecular mechanisms, substrate profiles, and therapeutic potential. Despite progress, challenges persist in fully characterizing its biological contexts, underscoring the continued need for high-quality reagents like FBXO34 antibodies in both basic and translational research.
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