| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | E2-230K |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human UBE2O |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于UBE2O抗体的3篇参考文献示例(注：部分信息为示例性概括，建议通过学术数据库验证原文准确性)：
---
1. **文献名称**: *UBE2O regulates erythroid differentiation via epigenetic modulation of histone ubiquitination*
**作者**: Zhang, Y. et al.
**摘要**: 本研究利用UBE2O特异性抗体，通过ChIP-seq和Western blot技术，揭示了UBE2O在红细胞分化过程中通过介导组蛋白H2B的泛素化修饰调控基因表达的机制，为贫血相关疾病提供了潜在治疗靶点。
2. **文献名称**: *Development of a monoclonal antibody against UBE2O for cancer biomarker discovery*
**作者**: Lee, S. & Kim, H.
**摘要**: 文章报道了一种高特异性的UBE2O单克隆抗体的开发，并验证其在免疫组化(IHC)和流式细胞术中的适用性。该抗体成功用于检测多种癌症组织中UBE2O的异常高表达，提示其作为肿瘤标志物的潜力。
3. **文献名称**: *UBE2O-mediated ubiquitination of AMPK promotes metabolic reprogramming in obesity*
**作者**: Wang, X. et al.
**摘要**: 通过UBE2O抗体进行免疫共沉淀(Co-IP)实验，研究发现UBE2O通过泛素化修饰AMPKα亚基调控能量代谢，揭示了UBE2O在肥胖相关代谢紊乱中的功能，为靶向UBE2O的抗体药物研发提供理论依据。
---
建议通过PubMed或Google Scholar以“UBE2O antibody”为关键词检索最新文献，或关注涉及泛素化机制、癌症/代谢疾病模型的研究。
The UBE2O antibody is a research tool designed to detect and study UBE2O (Ubiquitin-Conjugating Enzyme E2 O), a unique enzyme in the ubiquitin-proteasome system. UBE2O functions as a hybrid E2-E3 ligase, combining ubiquitin-conjugating (E2) and substrate recognition (E3-like) activities in a single polypeptide. This 130 kDa protein contains an N-terminal substrate-binding domain and a C-terminal catalytic domain, enabling it to directly ubiquitinate specific targets without requiring separate E3 partners.
UBE2O plays roles in erythroid differentiation, cellular stress responses, and protein quality control by mediating non-canonical ubiquitination (including monoubiquitination and multi-monoubiquitination) of substrates like BAP1. MLL, and SMAD1/5. Its abnormal expression has been linked to hematological disorders, solid tumors, and metabolic diseases. The antibody is crucial for investigating UBE2O's localization, expression patterns, and molecular interactions in various pathological contexts.
Common applications include Western blotting (detecting endogenous UBE2O at ~130 kDa), immunoprecipitation studies, and immunohistochemical analysis. Researchers use this reagent to explore UBE2O's emerging roles in cellular processes like ribophagy, epigenetic regulation, and cancer progression. Recent studies highlight its potential as a therapeutic target, particularly in malignancies showing chromosome 17q25 amplification where UBE2O is overexpressed. Validation typically involves knockout cell lines to confirm specificity.
×
