| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NOP5; HSPC120; NOP5/NOP58 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NOP58 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NOP58抗体的3篇参考文献示例(文献信息为模拟概括，仅供参考)：
1. **文献名称**：NOP58 interacts with dyskerin and regulates telomerase RNA localization in human cells
**作者**：Smith A, et al.
**摘要**：研究利用NOP58抗体进行免疫共沉淀实验，揭示了NOP58与dyskerin蛋白的相互作用，并证明其在端粒酶RNA亚细胞定位中的关键作用，为端粒相关疾病机制提供新见解。
2. **文献名称**：Role of NOP58 in ribosome biogenesis and its dysregulation in colorectal cancer
**作者**：Wang Y, et al.
**摘要**：通过NOP58抗体的Western blot和免疫组化分析，发现NOP58在结直肠癌中表达异常升高，其缺失导致rRNA加工缺陷，提示其可能作为癌症治疗的潜在靶点。
3. **文献名称**：Structural insights into the assembly of box C/D snoRNP complexes by NOP58 antibody-based cryo-EM
**作者**：Li H, et al.
**摘要**：结合NOP58抗体的冷冻电镜技术，解析了C/D box snoRNP复合物的三维结构，阐明了NOP58在复合物组装中的构象变化及RNA结合位点。
*注：以上为模拟文献，实际研究需通过PubMed等数据库检索(关键词：NOP58 antibody + 研究领域)。如需真实文献，建议补充具体研究背景或应用场景。*
NOP58 antibody is a research tool targeting the NOP58 protein, a core component of the C/D box small nucleolar ribonucleoprotein (C/D snoRNP) complex. This evolutionarily conserved complex plays a critical role in ribosomal RNA (rRNA) biogenesis by guiding site-specific 2'-O-methylation, a post-transcriptional modification essential for ribosome assembly and function. NOP58 interacts with other core proteins (e.g., fibrillarin, NOP56. and SNU13) and snoRNAs to form functional complexes that direct methylation to target rRNA sites.
As a molecular marker, NOP58 antibody is widely used in studies investigating nucleolar organization, ribosomopathies, and cellular stress responses. It helps detect NOP58 expression levels, subcellular localization (primarily nucleolar), and protein-protein interactions via techniques like Western blot, immunofluorescence, and immunoprecipitation. Dysregulation of NOP58 has been implicated in diseases linked to defective ribosome biogenesis, including certain cancers and genetic disorders like Bowen-Conradi syndrome. Researchers also utilize this antibody to explore connections between rRNA processing defects and cellular pathways such as p53 activation. Commercial NOP58 antibodies are typically raised in rabbits or mice against conserved epitopes, with validation across human and model organisms.
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