Rabbit Polyclonal P4HA2 Antibody

The P4HA2 antibody is a tool used to detect prolyl 4-hydroxylase subunit alpha 2 (P4HA2), a critical enzyme in collagen biosynthesis. P4HA2. part of the prolyl 4-hydroxylase family, catalyzes the hydroxylation of proline residues in collagen precursors, a post-translational modification essential for stabilizing collagen's triple-helical structure. This process ensures proper collagen deposition and extracellular matrix (ECM) remodeling, impacting tissue integrity and function. Dysregulation of P4HA2 is linked to fibrotic disorders, tumor progression, and metastasis, as cancer-associated fibroblasts often upregulate P4HA2 to enhance ECM stiffness, promoting tumor invasion. The P4HA2 antibody is widely used in research to study collagen-related pathologies, including cancer, fibrosis, and wound healing. It enables detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping to map P4HA2 expression patterns in tissues or cellular models. Recent studies also explore its role in hypoxia adaptation, as collagen hydroxylation influences ECM signaling under low-oxygen conditions. Commercially available monoclonal or polyclonal P4HA2 antibodies vary in specificity and host species, requiring validation for experimental conditions. Understanding P4HA2's function through antibody-based assays offers insights into therapeutic targeting of ECM dysregulation in disease.