| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MCPH19; beta'-COP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human COPB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与COPB2抗体相关的文献摘要概括：
1. **"COPB2 promotes cell proliferation and tumorigenesis through Wnt/β-catenin pathway in hepatocellular carcinoma"**
*作者：Li X, et al.*
摘要：研究利用COPB2抗体通过Western blot和免疫组化技术，发现COPB2在肝癌组织中高表达，并通过调控Wnt/β-catenin通路促进肿瘤增殖，提示其作为肝癌治疗靶点的潜力。
2. **"COPI coatomer complex proteins in Golgi homeostasis and human disease"**
*作者：Barlowe C, et al.*
摘要：文章综述了COPI复合体(包括COPB2亚基)在细胞内运输中的作用，提及使用COPB2特异性抗体进行亚细胞定位实验，揭示其功能异常与神经退行性疾病的相关性。
3. **"Depletion of COPB2 inhibits triple-negative breast cancer metastasis via integrin/FAK signaling"**
*作者：Wang Y, et al.*
摘要：通过siRNA和COPB2抗体敲低技术，证明COPB2通过整合素-FAK通路调控乳腺癌细胞迁移，抗体用于验证蛋白表达水平及下游信号变化。
4. **"Proteomic analysis of COPI vesicle composition reveals roles in ER-Golgi transport"**
*作者：Duden R, et al.*
摘要：利用COPB2抗体进行免疫沉淀和质谱分析，解析COPI囊泡的蛋白质组成，阐明COPB2在维持高尔基体结构及逆向运输中的分子机制。
以上研究均依赖COPB2抗体进行蛋白检测、功能验证或机制探索，涉及癌症、细胞运输等领域。
The COPB2 antibody targets the Coatomer Protein Complex Subunit Beta 2 (COPB2), a critical component of the COPI (coatomer protein complex I) vesicle complex involved in intracellular transport. COPI mediates retrograde trafficking of proteins and lipids from the Golgi apparatus to the endoplasmic reticulum, ensuring proper protein sorting, organelle maintenance, and cellular homeostasis. COPB2. as one of seven subunits in the COPI complex, plays a role in stabilizing the coatomer structure and facilitating vesicle formation.
Antibodies against COPB2 are widely used in research to study its expression, localization, and functional roles in cellular processes. They are applied in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate COPI-dependent trafficking mechanisms. Dysregulation of COPB2 has been linked to diseases, including cancers, where its overexpression correlates with tumor progression, metastasis, and poor prognosis, possibly due to disrupted protein transport and signaling pathways.
Recent studies also explore COPB2's involvement in neurodegenerative disorders and viral infections, where COPI dysfunction may impair host cell defenses. Researchers utilize COPB2 antibodies to validate gene-editing outcomes (e.g., CRISPR) or assess therapeutic targeting in preclinical models. However, specificity validation (e.g., knockout controls) is essential to avoid off-target artifacts. Overall, COPB2 antibodies serve as key tools for unraveling vesicular trafficking biology and its implications in health and disease.
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