| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLD4 |
| Uniprot No | Q9HCU8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-107aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是围绕POLD4重组蛋白相关研究的示例性参考文献(内容为模拟概括，实际引用需核实原文)：
---
1. **标题**: *POLD4 Deficiency Impairs DNA Polymerase Delta Activity and Promotes Genomic Instability*
**作者**: H. Wang et al.
**摘要**: 本研究通过重组POLD4蛋白与DNA聚合酶δ其他亚基的共表达，发现POLD4是维持该酶全活性的关键组分。实验表明，POLD4缺失会导致DNA复制保真度下降，并增加细胞对DNA损伤因子的敏感性，提示其在基因组稳定性中的作用。
2. **标题**: *Structural Insights into the Role of POLD4 in the DNA Polymerase Delta Complex*
**作者**: J. Zhang et al.
**摘要**: 通过冷冻电镜解析了包含重组POLD4蛋白的人源DNA聚合酶δ四聚体结构，揭示了POLD4通过C端结构域与其他亚基互作，调控酶复合物的装配与功能，为理解其分子机制提供结构基础。
3. **标题**: *Overexpression of Recombinant POLD4 Enhances Chemoresistance in Cancer Cells*
**作者**: K. Lee et al.
**摘要**: 在结直肠癌细胞中过表达重组POLD4蛋白，发现其通过加速损伤DNA的复制重启，降低化疗药物(如顺铂)的杀伤效果，表明POLD4可能成为癌症治疗耐药性的潜在靶点。
---
**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Web of Science等数据库检索真实文献并核对细节。
**Background of POLD4 Recombinant Protein**
POLD4 (DNA Polymerase Delta Subunit 4), also known as PolD4 or p12. is a critical regulatory subunit of the DNA polymerase delta (Pol δ) complex, a key enzyme in eukaryotic DNA replication and repair. The Pol δ holoenzyme consists of four subunits (POLD1. POLD2. POLD3. and POLD4) and plays essential roles in leading-strand synthesis, base excision repair, and genome stability maintenance. POLD4. the smallest subunit, modulates Pol δ activity and stability, though its precise mechanistic role remains under investigation.
Recombinant POLD4 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The protein is purified via affinity chromatography, often tagged with His or GST for isolation. Its recombinant form enables functional studies to dissect Pol δ's structure-function relationships, particularly in DNA replication fidelity, cell cycle regulation, and responses to replication stress.
Research on POLD4 has gained attention due to its implications in cancer and aging. Dysregulation of Pol δ subunits, including POLD4. is linked to genomic instability, tumorigenesis, and chemoresistance. Recombinant POLD4 facilitates *in vitro* assays to screen inhibitors targeting Pol δ for anticancer therapies. Additionally, studies explore how POLD4 depletion affects replication fork dynamics and DNA damage response pathways.
Despite its smaller size, POLD4's role in stabilizing the Pol δ complex highlights its biological significance. Ongoing work aims to clarify its contributions to DNA repair mechanisms and its potential as a biomarker or therapeutic target. Recombinant POLD4 thus serves as a vital tool for advancing genomic stability research and precision medicine applications.
×
