| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | RAY, H-ray, RAB1C |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human RAB35 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RAB35抗体的模拟参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**: *RAB35 regulates exosome-mediated cell-cell communication in cancer metastasis*
**作者**: Smith J, et al.
**摘要**: 本研究利用RAB35特异性抗体，揭示了RAB35通过调控外泌体释放促进肿瘤细胞迁移的分子机制，证明其与EGFR信号通路的相互作用。
2. **文献名称**: *Antibody-based targeting of RAB35 rescues synaptic defects in Alzheimer's models*
**作者**: Chen L, et al.
**摘要**: 通过RAB35抗体干预，研究者发现抑制RAB35活性可改善阿尔茨海默病模型中的突触功能障碍，提示其作为神经退行性疾病治疗靶点的潜力。
3. **文献名称**: *RAB35 antibody screening identifies novel roles in cytokinesis and membrane trafficking*
**作者**: Tanaka K, et al.
**摘要**: 利用多种RAB35单克隆抗体进行功能筛选，发现RAB35通过调控内吞循环通路影响细胞分裂末期膜分离过程，并揭示其与ARP2/3复合物的关联。
4. **文献名称**: *Development of a high-affinity RAB35 monoclonal antibody for diagnostic applications*
**作者**: Wang Y, et al.
**摘要**: 报道一种新型RAB35单克隆抗体的开发，该抗体在免疫组化中表现出高特异性，成功应用于胃癌组织样本中RAB35过表达检测及预后评估。
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注：以上为模拟案例，真实文献需通过PubMed、Google Scholar等平台以"RAB35 antibody"或"RAB35 function"为关键词检索。
Rab35 is a member of the Rab GTPase family, which regulates intracellular vesicle trafficking, membrane dynamics, and organelle organization. As a small GTP-binding protein, Rab35 cycles between active GTP-bound and inactive GDP-bound states, mediated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). It plays critical roles in endocytic recycling, cytokinesis, neurite outgrowth, and receptor signaling. Dysregulation of Rab35 is linked to cancer progression, neurodevelopmental disorders, and immune response defects.
Rab35 antibodies are essential tools for studying its expression, localization, and function. These antibodies, often produced in rabbits or mice, target specific epitopes of Rab35 for applications like Western blotting, immunofluorescence, immunoprecipitation, and flow cytometry. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect diverse isoforms. Validated antibodies help assess Rab35’s involvement in cellular processes or diseases by identifying overexpression, phosphorylation, or interaction partners.
Research using Rab35 antibodies has revealed its role in exosome secretion, autophagy, and cytoskeletal remodeling. Commercial antibodies typically undergo validation via knockout cell lines or siRNA knockdown to confirm target specificity. Proper controls, like isotype-matched IgG or lysates from Rab35-deficient models, are critical to avoid off-target artifacts. Overall, Rab35 antibodies are pivotal in elucidating its contributions to cellular homeostasis and pathology.
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