Rabbit Polyclonal PRKAA1 Antibody

The PRKAA1 antibody is a crucial tool for studying the alpha-1 catalytic subunit of adenosine monophosphate-activated protein kinase (AMPK), a central regulator of cellular energy homeostasis. AMPK, a heterotrimeric enzyme, senses metabolic stress and coordinates pathways that restore ATP levels by inhibiting anabolic processes (e.g., lipid synthesis) and promoting catabolic activities (e.g., glucose uptake, fatty acid oxidation). PRKAA1 (also known as AMPKα1) is ubiquitously expressed and plays a key role in metabolic diseases, cancer, and aging. Antibodies targeting PRKAA1 enable researchers to detect its expression, phosphorylation status (e.g., at Thr172 for activation), and subcellular localization via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These applications are vital for investigating AMPK signaling in response to stressors like nutrient deprivation, exercise, or pharmacological agents (e.g., metformin). PRKAA1-specific antibodies are also used to explore tissue-specific AMPK functions, such as its role in liver gluconeogenesis, muscle glucose metabolism, or neuronal survival. Validated antibodies with high specificity (confirmed via knockout controls or siRNA knockdown) are essential to avoid cross-reactivity with the homologous alpha-2 subunit (PRKAA2). Research utilizing PRKAA1 antibodies has advanced understanding of metabolic syndromes, cancer metabolism, and therapeutic targeting of AMPK pathways.