| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | ANPa, NPRA, ANPRA, GUC2A, GUCY3A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NPR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NPR1抗体的3篇参考文献及其摘要内容概括：
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1. **文献名称**: *The Arabidopsis NPR1 Gene That Controls Systemic Acquired Resistance Encodes a Novel Protein Containing Ankyrin Repeats*
**作者**: Cao, H., Glazebrook, J., Clarke, J.D., Volko, S., Dong, X.
**摘要**: 该研究首次克隆并鉴定了拟南芥NPR1基因，发现其编码含有锚蛋白重复序列的蛋白，调控系统获得性抗性(SAR)。研究中通过制备特异性抗体，证实NPR1蛋白在病原体感染后积累，且其表达水平与抗病性正相关。
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2. **文献名称**: *Inducers of Plant Systemic Acquired Resistance Regulate NPR1 Function through Redox Changes*
**作者**: Mou, Z., Fan, W., Dong, X.
**摘要**: 文章揭示了水杨酸(SA)通过调控细胞氧化还原状态激活NPR1蛋白的分子机制。利用NPR1特异性抗体进行亚细胞定位分析，发现SA诱导NPR1从寡聚体解离为单体，并转运至细胞核启动抗病基因表达。
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3. **文献名称**: *Proteasome-Mediated Turnover of the Transcription Coactivator NPR1 Plays Dual Roles in Regulating Plant Immunity*
**作者**: Spoel, S.H., Mou, Z., Tada, Y., Loake, G.J., Dong, X.
**摘要**: 本研究通过免疫印迹和抗体检测，发现NPR1的稳定性受26S蛋白酶体调控。病原体感染后，NPR1的磷酸化修饰促进其降解以终止免疫响应，而SA则通过抑制降解维持其活性，揭示了NPR1动态调控的双重作用。
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如需更多文献或特定应用场景的抗体研究，可进一步补充关键词细化检索。
The NPR1 (Non-Expresser of PR Genes 1) antibody is a crucial tool for studying plant immune responses, particularly in the model organism *Arabidopsis thaliana*. NPR1 is a central regulatory protein in systemic acquired resistance (SAR), a long-lasting defense mechanism activated by pathogens. It functions as a salicylic acid (SA) receptor, mediating SA-dependent signaling to induce pathogenesis-related (PR) genes. Structurally, NPR1 contains ankyrin repeats and a BTB/POZ domain, facilitating protein-protein interactions.
Researchers developed NPR1 antibodies to investigate its localization, expression dynamics, and interaction partners. These antibodies enable detection of NPR1 via techniques like Western blot, immunoprecipitation, and immunohistochemistry. Studies using NPR1 antibodies revealed its redox-sensitive nuclear translocation and role in coordinating defense responses with growth processes.
NPR1 homologs exist across plant species, making these antibodies valuable for comparative studies in crop disease resistance. Mutant analyses (e.g., *npr1* knockouts) combined with antibody-based approaches have clarified its necessity for SAR establishment. Recent work explores NPR1's crosstalk with jasmonic acid pathways and its post-translational modifications. Commercial NPR1 antibodies are typically raised against conserved epitopes, often validated in genetic mutants to ensure specificity. Their applications span basic research in plant-pathogen interactions to biotechnological efforts enhancing crop resilience.
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