| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | GPBB |
| WB Predicted band size | 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PYGB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于公开信息的示例性参考文献(注：以下内容为模拟概括，实际文献可能需要通过学术数据库查询)：
1. **文献名称**：*"PYGB promotes glioblastoma progression by regulating glucose metabolism"*
**作者**：Smith A, et al.
**摘要**：研究利用PYGB抗体分析脑胶质瘤组织中PYGB蛋白表达，发现其高表达与肿瘤细胞糖酵解增强及患者预后不良相关，提示PYGB可能成为治疗靶点。
2. **文献名称**：*"Hepatocellular carcinoma progression relies on glycogen phosphorylase B (PYGB) activity"*
**作者**：Chen L, et al.
**摘要**：通过免疫组化(PYGB抗体)和功能实验，证实PYGB在肝癌中通过调控糖原分解促进肿瘤细胞增殖和侵袭。
3. **文献名称**：*"Antibody-based inhibition of PYGB mitigates ischemic cardiac injury"*
**作者**：Zhang Y, et al.
**摘要**：研究使用特异性PYGB抗体抑制心肌细胞PYGB活性，发现可减少缺血再灌注损伤，为心脏保护策略提供新方向。
4. **文献名称**：*"Tissue-specific expression of PYGB in metabolic disorders"*
**作者**：Kim J, et al.
**摘要**：通过Western blot(PYGB抗体)分析糖尿病模型小鼠组织，发现PYGB在肝脏和肌肉中异常表达，可能与胰岛素抵抗相关。
**建议**：通过PubMed或Google Scholar检索“PYGB antibody”或“glycogen phosphorylase B”+“specific disease”获取真实文献。
PYGB antibody is a research tool targeting the brain isoform of glycogen phosphorylase (PYGB), a key enzyme in glycogen metabolism. Glycogen phosphorylase catalyzes the rate-limiting step of glycogenolysis, breaking down glycogen into glucose-1-phosphate to meet cellular energy demands. The PYGB isoform is predominantly expressed in the brain, heart, and fetal liver, distinguishing it from the muscle (PYGM) and liver (PYGL) isoforms. Its activity is regulated by allosteric effectors and phosphorylation, linking cellular energy status to glycogen utilization.
PYGB's role in maintaining energy homeostasis is particularly critical in tissues with high metabolic demands, such as neurons. Dysregulation of PYGB has been implicated in glycogen storage diseases, neurodegenerative disorders, and certain cancers. For example, reduced PYGB activity may contribute to abnormal glycogen accumulation in Alzheimer’s disease, while its overexpression has been observed in glioblastoma, suggesting a potential therapeutic target.
Antibodies against PYGB are widely used in biomedical research to investigate tissue-specific glycogen metabolism, disease mechanisms, and protein localization. They enable detection of PYGB expression levels via Western blotting, immunohistochemistry, and immunofluorescence. Recent studies also explore PYGB's interplay with hypoxia pathways and metabolic reprogramming in tumors. Validation of PYGB antibody specificity remains essential, given the high sequence homology between phosphorylase isoforms. Ongoing research continues to clarify PYGB's pathophysiological significance across neurological and oncological contexts.
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