Rabbit Polyclonal RAB5A Antibody

Rab5A antibody is a valuable tool in cellular and molecular biology research, targeting the Rab5A protein, a member of the Rab GTPase family. Rab5A plays a critical role in regulating early endosome dynamics, membrane trafficking, and vesicular transport during endocytosis. It cycles between GTP-bound (active) and GDP-bound (inactive) states, facilitating the fusion of plasma membrane-derived vesicles with early endosomes and coordinating cargo sorting. Dysregulation of Rab5A has been implicated in pathological processes, including cancer metastasis, neurodegenerative disorders, and viral entry mechanisms. Researchers use Rab5A antibodies to detect and quantify Rab5A expression in various experimental models (e.g., cell lines, tissues) via techniques like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies help elucidate Rab5A's subcellular localization, interaction partners, and functional alterations under different physiological or disease conditions. Commercially available Rab5A antibodies are typically raised in host species like rabbits or mice, with validation data confirming specificity through knockout controls or siRNA knockdown. Some antibodies distinguish between active (GTP-bound) and total Rab5A, enabling studies on its activation status. Optimal performance depends on proper sample preparation, antibody dilution, and experimental controls to avoid cross-reactivity with homologous proteins (e.g., Rab5B/C).