| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARF6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARF6抗体的3篇代表性文献，包含文献名称、作者及摘要内容概述：
1. **文献名称**：*ARF6 regulates endocytic recycling and actin cytoskeleton remodeling*
**作者**：Donaldson, J.G. et al.
**摘要**：该研究利用ARF6特异性抗体，通过免疫印迹和免疫荧光技术，揭示ARF6通过调控内吞循环和肌动蛋白重组，影响细胞迁移和肿瘤侵袭的分子机制。
2. **文献名称**：*ARF6 promotes axonal growth through regulation of N-cadherin trafficking*
**作者**：Schlüter, O.M. et al.
**摘要**：研究通过ARF6抗体进行免疫共沉淀实验，发现ARF6通过介导N-钙粘蛋白的膜运输，促进神经元轴突生长，为神经发育提供新见解。
3. **文献名称**：*ARF6 modulates TLR4 signaling to control bacterial phagocytosis*
**作者**：Radhakrishna, H. et al.
**摘要**：文章使用ARF6抗体进行功能阻断实验，证明ARF6通过调控TLR4信号通路，影响巨噬细胞对细菌的吞噬能力，关联天然免疫应答机制。
这些研究均依赖ARF6抗体的特异性检测，分别在细胞运输、神经发育及免疫领域阐明了ARF6的生物学功能。
ADP-ribosylation factor 6 (ARF6) is a small GTP-binding protein belonging to the ARF family, which regulates membrane trafficking, cytoskeletal reorganization, and cellular signaling. As a key regulator of endocytic and exocytic pathways, ARF6 controls the recycling of plasma membrane components, cell adhesion, and migration. Antibodies targeting ARF6 are essential tools for studying its spatial-temporal dynamics, activation states (GTP- or GDP-bound), and interactions with effector proteins like JIP3. PLD, or GGA3 in diverse cellular processes.
ARF6 antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect its expression, subcellular localization (plasma membrane, endosomes, or tubular vesicles), and activity-dependent redistribution. These antibodies help elucidate ARF6's roles in physiological contexts (e.g., neurite outgrowth, immune response) and pathological conditions, including cancer metastasis, viral infection, and neurological disorders. Commercial ARF6 antibodies are often raised against specific epitopes, such as the N-terminal region (residues 2-175) or C-terminal domains, with validation in knockout models to ensure specificity. Recent studies using ARF6 antibodies have highlighted its involvement in tumor invasion, exosome secretion, and β-amyloid production in Alzheimer’s disease, making it a potential therapeutic target. Researchers prioritize antibodies recognizing both inactive and active conformations to dissect ARF6-mediated molecular mechanisms.
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