| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | AMPKG |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human PRKAG1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRKAG1抗体的3篇参考文献，涵盖其在不同研究中的应用和发现：
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1. **文献名称**: *"PRKAG1 Cardiac Syndrome: Familial Ventricular Preexcitation, Conduction System Disease, and Cardiac Hypertrophy"*
**作者**: Gollob MH 等
**摘要**: 该研究通过Western blot和免疫组化分析PRKAG1在心脏组织中的表达，发现其突变导致AMPK γ1亚基功能异常，引发心室预激、传导系统疾病及心脏肥大。研究利用特异性抗体证实突变蛋白的异常积累与病理相关。
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2. **文献名称**: *"AMPK γ1 subunit mutations modulate glucose uptake in skeletal muscle"*
**作者**: Barnes BR 等
**摘要**: 研究使用PRKAG1抗体检测小鼠骨骼肌中AMPK γ1的亚细胞定位，发现其突变影响AMPK活性，导致葡萄糖转运蛋白GLUT4的膜转位异常，揭示了其在能量代谢调控中的作用。
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3. **文献名称**: *"Overexpression of PRKAG1 in hepatocellular carcinoma promotes tumor progression via AMPK signaling"*
**作者**: Li Y 等
**摘要**: 通过免疫组化和Western blot分析肝癌组织样本，发现PRKAG1在肿瘤中高表达，且与患者不良预后相关。抗体实验证实其通过激活AMPK-mTOR通路促进肿瘤细胞增殖和侵袭。
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如需更多文献或特定研究方向的补充，可进一步说明。
The PRKAG1 antibody is a crucial tool for studying the gamma-1 subunit of the AMP-activated protein kinase (AMPK), a central regulator of cellular energy homeostasis. AMPK, a heterotrimeric enzyme, consists of alpha (catalytic), beta, and gamma subunits. PRKAG1 encodes the gamma-1 subunit, which binds adenosine nucleotides (AMP, ADP, ATP) to modulate AMPK activity in response to metabolic stress. This subunit plays a vital role in sensing energy depletion, triggering downstream pathways that restore ATP levels by inhibiting anabolic processes and promoting catabolic reactions like glucose uptake and fatty acid oxidation. Dysregulation of PRKAG1 is linked to metabolic disorders, including diabetes, obesity, and cardiovascular diseases.
PRKAG1 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They help investigate AMPK's role in metabolic signaling, cancer progression (where AMPK acts as a tumor suppressor or promoter), and cardiac hypertrophy associated with PRKAG1 mutations. Specificity validation (e.g., knockout controls) is essential due to potential cross-reactivity with other gamma subunits (PRKAG2. PRKAG3). Commercial PRKAG1 antibodies are typically raised in rabbits or mice, targeting epitopes within conserved regions of the human protein. Their application advances understanding of metabolic regulation and therapeutic targeting of AMPK-related pathologies.
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