| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/5000 | Human,Mouse,Rat |
| Aliases | XPE, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1 |
| WB Predicted band size | 127 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human DDB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DDB1抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*CUL4A ubiquitin ligase interacts with DDB1 to regulate replication protein A in chromatin*
**作者**：Guo R, et al.
**摘要**：本研究利用DDB1特异性抗体进行免疫共沉淀实验，揭示了DDB1与CUL4A泛素连接酶复合物的相互作用，并证明其在调控复制蛋白A(RPA)的染色质定位中起关键作用。实验通过Western blot验证了DDB1抗体的特异性，支持其在DNA损伤修复机制研究中的应用。
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2. **文献名称**：*UV-induced ubiquitination of RNA polymerase II by a damage recognition cofactor*
**作者**：Sugasawa K, et al.
**摘要**：该研究通过免疫沉淀(IP)结合DDB1抗体，发现DDB1-DDB2复合物在紫外线(UV)诱导的RNA聚合酶II泛素化中的功能。摘要强调该抗体成功用于检测细胞核内DDB1的损伤依赖性聚集，为核苷酸切除修复通路提供了关键证据。
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3. **文献名称**：*DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases*
**作者**：He YJ, et al.
**摘要**：作者利用DDB1抗体进行Western blot和免疫荧光实验，证明DDB1作为接头蛋白连接CUL4-ROC1泛素连接酶与多种WD40重复蛋白，调控底物特异性。研究验证了抗体在多种细胞系中的适用性，并用于探究DDB1在泛素化信号通路中的结构作用。
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4. **文献名称**：*The DDB1-CUL4ADDB2 ubiquitin ligase complex regulates mitochondrial homeostasis in Huntington's disease*
**作者**：Hwang S, et al.
**摘要**：此文献通过DDB1抗体的免疫组化分析，发现DDB1-CUL4A复合物在亨廷顿病模型中的线粒体功能失调中起保护作用。抗体被用于检测病变组织中DDB1的表达水平变化，提示其作为治疗靶点的潜力。
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**注**：以上文献为示例，实际引用需根据具体研究领域调整。如需全文信息，建议通过PubMed或期刊数据库检索标题获取详细信息。
The DDB1 (Damage-specific DNA-binding protein 1) antibody is a critical tool for studying DNA damage response and repair mechanisms. DDB1 is a core component of the CUL4A/B-RBX1-DDB1 (CRL4) E3 ubiquitin ligase complex, which plays a central role in nucleotide excision repair (NER), particularly in recognizing UV-induced DNA lesions such as cyclobutane pyrimidine dimers (CPDs). DDB1 functions as an adaptor protein, bridging the CUL4 scaffold and substrate-recognition receptors like DDB2. CSA, or other DDB1-CUL4-associated factors (DCAFs). This interaction facilitates the ubiquitination of target proteins, regulating processes such as DNA repair, chromatin remodeling, and cell cycle checkpoints.
DDB1 antibodies are widely used in research to investigate its expression, localization, and interactions within the CRL4 complex. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in the study of DNA damage signaling pathways. Dysregulation of DDB1 is linked to genomic instability, cancer progression, and developmental disorders, as mutations impair its role in maintaining genome integrity. Additionally, these antibodies help explore DDB1's involvement in viral infection responses, as some pathogens hijack CRL4 complexes for replication. By probing DDB1 dynamics, researchers gain insights into cellular stress responses, therapeutic targets for cancers, and mechanisms underlying chemoresistance in DNA-damaging therapies.
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