| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ALK-SMase |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human ENPP7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ENPP7抗体的代表性文献(信息已简化整理)：
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1. **文献名称**: "Characterization of a monoclonal antibody against human intestinal alkaline sphingomyelinase (ENPP7)"
**作者**: Duan RD et al.
**摘要**: 报道了一种针对人ENPP7的单克隆抗体的开发，验证其在Western blot和免疫组化中的特异性，并发现ENPP7在小肠黏膜中高表达，提示其在肠道脂质代谢中的关键作用。
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2. **文献名称**: "ENPP7 regulates lipid metabolism in hepatocellular carcinoma via TGF-β/Smad signaling"
**作者**: Li Y et al.
**摘要**: 利用ENPP7抗体研究其在肝癌中的表达，发现ENPP7通过调控TGF-β通路影响鞘磷脂代谢，抑制肝癌细胞增殖，提示其作为潜在治疗靶点。
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3. **文献名称**: "Altered expression of alkaline sphingomyelinase (ENPP7) in colorectal cancer tissues"
**作者**: Cheng Y et al.
**摘要**: 通过ENPP7抗体的免疫组化分析，发现结直肠癌组织中ENPP7表达显著下调，与肿瘤进展和患者预后相关，提示其抑癌功能。
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注：以上文献信息为模拟示例，实际研究中建议通过PubMed或Google Scholar以“ENPP7 antibody”或“ENPP7 sphingomyelinase”为关键词检索最新文献。
The ENPP7 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 7) antibody is a tool used to study the ENPP7 protein, a member of the ectonucleotide pyrophosphatase/phosphodiesterase family. ENPP7. also known as alkaline sphingomyelinase, is a membrane-bound enzyme primarily expressed in the intestinal tract and liver. It hydrolyzes dietary sphingomyelin into ceramide and phosphocholine, playing a critical role in lipid metabolism and maintaining intestinal barrier function. Its activity is implicated in regulating cell proliferation, inflammation, and cholesterol absorption, with potential links to colorectal cancer, metabolic disorders, and inflammatory bowel diseases.
ENPP7 antibodies are widely utilized in research to detect protein expression, localization, and function via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate ENPP7's physiological roles and dysregulation in pathologies. Recent studies highlight ENPP7's involvement in gut microbiota interactions and its potential as a biomarker for gastrointestinal cancers. However, antibody specificity remains a challenge due to structural similarities within the ENPP family. Validated antibodies are essential for distinguishing ENPP7 from homologous proteins like ENPP1-3. Ongoing research aims to refine antibody performance and explore therapeutic targeting of ENPP7 in metabolic or neoplastic diseases.
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