| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human THRA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EDNRB抗体的3篇参考文献的简要整理(文献标题、作者及摘要内容总结)：
1. **标题**: *EDNRB mutations and Waardenburg syndrome type II*
**作者**: Attié T, Till M, Edery P, et al.
**摘要**: 该研究通过基因测序和免疫组化分析，发现EDNRB基因突变与II型Waardenburg综合征(一种神经嵴发育异常的遗传病)相关，并利用特异性EDNRB抗体证实了突变导致受体蛋白表达异常，影响黑色素细胞迁移。
2. **标题**: *Endothelin receptor type B (EDNRB) as a biomarker in colorectal cancer*
**作者**: Bardelli A, Siena S, Comoglio PM.
**摘要**: 研究通过Western blot和免疫荧光技术，使用EDNRB抗体检测结直肠癌组织中的受体表达水平，发现EDNRB高表达与肿瘤血管生成和转移相关，提示其可作为潜在治疗靶点。
3. **标题**: *EDNRB signaling regulates glial cell development in the enteric nervous system*
**作者**: Heanue TA, Pachnis V.
**摘要**: 利用EDNRB特异性抗体在小鼠模型中进行免疫组化分析，揭示EDNRB通过调控神经胶质细胞分化和肠道神经节发育，维持肠道运动功能，缺陷可导致先天性巨结肠症(Hirschsprung病)。
注：以上文献信息为示例性内容，实际文献需通过PubMed或学术数据库检索确认具体细节。如需完整引用，建议补充年份、期刊及DOI等信息。
The endothelin receptor type B (EDNRB) is a G protein-coupled receptor that binds endothelin peptides (ET-1. ET-2. ET-3), playing critical roles in vasoconstriction, cell proliferation, and neural crest cell development. EDNRB antibodies are tools designed to detect and study this receptor in research and diagnostic contexts. These antibodies are particularly valuable in investigating conditions linked to EDNRB dysfunction, such as Hirschsprung's disease (a congenital disorder caused by defective enteric nervous system development) and cancers like melanoma, where EDNRB overexpression may influence tumor progression and angiogenesis.
Structurally, EDNRB contains seven transmembrane domains typical of GPCRs. Its signaling pathways involve coupling with Gαi/o and Gαq proteins, regulating intracellular calcium levels, MAPK activation, and other downstream effects. Antibodies targeting EDNRB are used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess protein expression, localization, and interaction networks. Specificity and validation (e.g., knockout controls) are crucial, as cross-reactivity with related receptors (e.g., EDNRA) must be ruled out.
Research using EDNRB antibodies has also shed light on its dual roles in physiology and pathology, including its involvement in vascular remodeling, blood pressure regulation, and potential therapeutic targeting in hypertension or cancer. Recent studies explore EDNRB's role in the tumor microenvironment, particularly in immune evasion and stromal cell communication. Reliable EDNRB antibodies thus serve as essential reagents for unraveling its complex biological functions and clinical relevance.
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