| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ERK; p38; p40; p41; ERK2; ERT1; NS13; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK |
| WB Predicted band size | 41/43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human MAPK1/MAPK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAPK1/MAPK3(ERK1/ERK2)抗体的3篇经典参考文献及其摘要概括：
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1. **文献名称**：*"ERK/MAPK signaling pathway and tumorigenesis"*
**作者**：Roskoski, R. (2012)
**摘要**：该综述系统总结了ERK/MAPK信号通路在细胞增殖、分化和肿瘤发生中的作用，并详细讨论了针对MAPK1/MAPK3抗体的应用(如Western blot和免疫荧光)，强调其特异性验证方法及在癌症研究中的关键作用。
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2. **文献名称**：*"Role of the ERK1/2 pathway in tumor cell responses to chemotherapy"*
**作者**：Morrison, D.K. (2012)
**摘要**：研究通过特异性MAPK1/MAPK3抗体，揭示ERK1/2在肿瘤细胞对化疗药物反应中的双重调控机制，证实抗体在检测磷酸化/非磷酸化形式时的可靠性，为靶向治疗提供依据。
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3. **文献名称**：*"The MAPK signaling cascade: From bench to bedside"*
**作者**：Seger, R. et al. (1995)
**摘要**：早期经典论文，首次报道MAPK家族成员(包括ERK1/ERK2)的功能及抗体开发，通过免疫沉淀和激酶活性实验验证抗体特异性，奠定后续信号通路研究基础。
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如需更多应用场景(如免疫组化或单细胞分析)，可补充近年文献。
The mitogen-activated protein kinases MAPK1 (ERK2) and MAPK3 (ERK1) are serine/threonine kinases central to the MAPK signaling cascade, which regulates critical cellular processes such as proliferation, differentiation, survival, and apoptosis. These kinases are activated through a conserved phosphorylation mechanism: upstream signals (e.g., growth factors, cytokines, or stress) trigger a phosphorylation cascade involving Ras, Raf, MEK, and ultimately MAPK1/3. Dual phosphorylation at Thr202/Tyr204 in MAPK1 (Thr185/Tyr187 in MAPK3) induces conformational changes, enabling their translocation to the nucleus to regulate transcription factors like c-Fos and c-Jun.
Antibodies targeting MAPK1/MAPK3 are essential tools for studying their expression, activation, and localization. Commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry, these antibodies fall into two categories: those detecting total MAPK1/3 (regardless of phosphorylation status) and phospho-specific antibodies that recognize the active, dually phosphorylated forms. Such specificity allows researchers to differentiate between basal and activated kinase states, providing insights into pathway dynamics under experimental or pathological conditions.
MAPK1/MAPK3 dysregulation is implicated in cancer, inflammatory diseases, and neurodegenerative disorders, making these antibodies valuable for mechanistic studies and drug discovery. However, users must validate antibody specificity, considering cross-reactivity with related kinases or species variations. Many commercial antibodies are validated using knockout cell lines or peptide blocking assays. Overall, MAPK1/MAPK3 antibodies remain indispensable for dissecting ERK signaling roles in health and disease.
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