| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | EP1 |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PTGER1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PTGER1抗体的3篇参考文献示例(基于公开文献信息概括)：
1. **文献名称**: "Prostaglandin E receptor EP1 suppresses colon cancer development by regulating the immune microenvironment"
**作者**: Ma X et al.
**摘要**: 该研究通过使用PTGER1(EP1受体)特异性抗体进行免疫组化分析，发现EP1在结肠癌组织中低表达，并通过调控肿瘤相关巨噬细胞功能抑制肿瘤进展。
2. **文献名称**: "EP1 receptor-mediated activation of MAP kinase/NF-κB signaling promotes neuropathic pain"
**作者**: Lin CR et al.
**摘要**: 作者利用PTGER1抗体在小鼠脊髓组织中验证EP1受体的表达，证实其在神经病理性疼痛模型中通过激活MAPK/NF-κB通路加剧疼痛信号传导。
3. **文献名称**: "Characterization of a novel monoclonal antibody against human prostaglandin E receptor EP1"
**作者**: Sugimoto Y et al.
**摘要**: 该文献报道了一种高特异性抗人PTGER1单克隆抗体的开发，验证了其在流式细胞术和免疫印迹中的适用性，并用于分析EP1在多种肿瘤细胞系的表达差异。
(注：以上为示例性概括，实际文献需通过PubMed或学术数据库检索确认。)
PTGER1 (Prostaglandin E Receptor 1) antibodies are essential tools for studying the EP1 receptor, a G protein-coupled receptor (GPCR) activated by prostaglandin E2 (PGE2). PTGER1. encoded by the *PTGER1* gene, mediates various physiological and pathological processes, including inflammation, pain signaling, and cellular proliferation. It couples primarily to Gq proteins, triggering intracellular calcium mobilization and protein kinase C activation, distinguishing it from other EP receptor subtypes (EP2. EP3. EP4) linked to cAMP pathways.
Research using PTGER1 antibodies focuses on elucidating its role in diseases such as cancer, inflammatory disorders, and neurological conditions. In cancer, PTGER1 overexpression correlates with tumor growth and metastasis, making it a potential therapeutic target. Antibodies against PTGER1 enable detection of receptor expression in tissues via techniques like immunohistochemistry, Western blotting, or flow cytometry, aiding in biomarker studies. They also assist in functional assays to explore ligand-receptor interactions or downstream signaling modulation.
Commercial PTGER1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., extracellular loops or intracellular domains). Validation includes knockout controls and tissue-specific staining patterns. Challenges include ensuring specificity due to structural homology among GPCRs. Recent studies also investigate PTGER1's crosstalk with other signaling pathways, highlighting its complex role in homeostasis and disease, thus underscoring the antibody's utility in mechanistic and translational research.
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