Rabbit Polyclonal MAPK6 Antibody

The MAPK6 antibody is a crucial tool for studying the mitogen-activated protein kinase 6 (MAPK6), also known as extracellular signal-regulated kinase 3 (ERK3). MAPK6. a member of the atypical MAPK family, regulates diverse cellular processes, including proliferation, differentiation, stress responses, and cancer progression. Unlike canonical MAPKs (e.g., ERK1/2), MAPK6 lacks a dual phosphorylation motif in its activation loop and exhibits distinct regulatory mechanisms. The MAPK6 antibody specifically detects endogenous MAPK6 protein in various experimental applications, such as Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). It is often raised in rabbits or mice using immunogens derived from conserved regions or phosphorylation sites (e.g., Thr-207/Ser-209) of human MAPK6. Validation typically involves knockout controls or siRNA-mediated silencing to confirm specificity. Researchers utilize this antibody to explore MAPK6's role in signaling pathways, particularly its interaction with downstream effectors like MK5 (MAPKAPK5) and its implications in tumorigenesis, metastasis, and drug resistance. Cross-reactivity with homologous proteins (e.g., MAPK4) must be ruled out during optimization. As dysregulation of MAPK6 is linked to cancers, neurological disorders, and inflammation, this antibody aids in elucidating its pathophysiological relevance and therapeutic potential.