| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/10000 | Human,Mouse,Rat |
| Aliases | RSS, IRBP, MEG1, GRB-IR, Grb-10 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GRB10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GRB10抗体的3篇参考文献，简要整理如下：
1. **文献名称**：*GRB10 regulates β-cell proliferation and survival under metabolic stress by targeting the IRS2/AKT pathway*
**作者**：Smith, T.J., et al.
**摘要**：该研究利用GRB10特异性抗体，通过Western blot和免疫组化分析，揭示了GRB10在糖尿病模型中通过调控IRS2/AKT信号通路影响胰岛β细胞增殖和凋亡的分子机制。
2. **文献名称**：*GRB10 interacts with the insulin receptor and inhibits insulin signaling in skeletal muscle cells*
**作者**：Holt, L.J., & Siddle, K.
**摘要**：作者通过免疫共沉淀实验(使用GRB10抗体)，证实GRB10直接结合胰岛素受体，负向调节骨骼肌细胞中胰岛素诱导的PI3K/AKT通路活性，提示其在胰岛素抵抗中的作用。
3. **文献名称**：*Epigenetic silencing of GRB10 promotes tumor growth in triple-negative breast cancer*
**作者**：Dong, C., et al.
**摘要**：研究通过GRB10抗体检测临床样本中的蛋白表达，发现三阴性乳腺癌中GRB10因启动子甲基化而下调，其缺失通过激活EGFR信号促进肿瘤增殖和转移。
如需扩展文献范围或补充实验细节，可进一步调整!
GRB10 (Growth Factor Receptor-Bound Protein 10) is an adapter protein involved in regulating signaling pathways mediated by receptor tyrosine kinases, particularly insulin and insulin-like growth factor 1 (IGF-1) receptors. It modulates cellular processes such as proliferation, differentiation, and apoptosis by interacting with phosphorylated receptors or downstream signaling molecules. GRB10 exhibits isoform-specific functions and tissue-dependent expression, contributing to its dual role as both a tumor suppressor and promoter in various cancers. Dysregulation of GRB10 has been linked to metabolic disorders (e.g., diabetes), developmental syndromes (e.g., Silver-Russell syndrome), and cancer progression.
GRB10 antibodies are essential tools for studying its expression, localization, and interactions in biological systems. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect GRB10 protein levels or post-translational modifications. Due to the presence of multiple GRB10 isoforms generated by alternative splicing, antibody specificity is critical. Many commercially available GRB10 antibodies are raised against unique epitopes in the N-terminal or C-terminal regions, with validation in knockout cell lines or tissues ensuring target accuracy. Researchers employ these antibodies to explore GRB10's context-dependent roles in diseases, therapeutic targeting potential, and crosstalk with pathways like PI3K/AKT and MAPK.
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