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产品描述：马凝聚素(CLU)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CLU含量。CLU（Clusterin）是一种存在于多种细胞组分中的多功能蛋白，最早发现于山羊精液中，参与细胞间黏附。研究显示CLU与神经退行性疾病、免疫疾病、衰老和癌症等多种病理状态相关。CLU具有不同的蛋白产物，如分泌型（sCLU）和核型（nCLU），在细胞定位和生物学功能上有所不同。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，本产品适用于科研领域对CLU蛋白表达水平的精准分析，为研究CLU在心血管疾病、神经退行性病变及肿瘤微环境中的分子机制提供了可靠工具，也可用于评估实验动物模型或体外培养体系中CLU的干预调控效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLU ELISA Kit; Clusterin [Cleaved into: Clusterin beta chain; Clusterin alpha chain] ELISA Kit
缩写：CLU
Uniprot No.：Q29482
种属：Equus caballus (Horse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 101 | |||||
| Range % | 95-106 | ||||||
| 1:2000 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:4000 | Average % | 98 | |||||
| Range % | 92-102 | ||||||
| 1:8000 | Average % | 100 | |||||
| Range % | 94-104 | ||||||
回收率：
| The recovery of horse CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 86-96 | |||||
| EDTA plasma (n=4) | 102 | 95-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average10000.1710.1670.1695000.2770.2640.2712500.4980.4730.4861250.8110.8190.81562.51.3491.3301.34031.251.9061.8781.89215.62.4122.3352.37402.9502.8762.913 | |||||||
货期：3-5 working days
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