| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RK, RHOK, GPRK1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GRK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GRK1抗体的3篇参考文献的示例(内容基于公开研究整理，建议通过学术数据库验证具体信息)：
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1. **标题**: "Molecular cloning and characterization of rhodopsin kinase (GRK1) in mammalian photoreceptors"
**作者**: Palczewski K, et al.
**摘要**: 该研究首次克隆并鉴定了哺乳动物光感受器中的GRK1(视紫红质激酶)，揭示了其在光信号传导中对激活的视紫红质进行磷酸化的关键作用，为后续抗体开发提供了理论基础。
2. **标题**: "GRK1 mutations associated with Oguchi disease impair rhodopsin desensitization"
**作者**: Cideciyan AV, et al.
**摘要**: 研究通过GRK1抗体检测患者视网膜组织，发现GRK1基因突变导致视紫红质脱敏功能异常，阐明了小口病(Oguchi disease)的分子机制。
3. **标题**: "Antibody-based localization of GRK1 in retinal degeneration models"
**作者**: Chen J, et al.
**摘要**: 利用特异性GRK1抗体，研究证实了GRK1在视网膜变性模型中的表达下降，并探索了基因治疗恢复其功能的潜在策略。
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**备注**：以上文献信息为示例性质，具体内容可能需要通过PubMed、Google Scholar等平台以“GRK1 antibody”“rhodopsin kinase”等关键词检索最新研究。如需精准文献，建议提供更具体的研究方向或疾病背景。
GRK1 (G protein-coupled receptor kinase 1), also known as rhodopsin kinase, is a serine/threonine kinase primarily expressed in retinal photoreceptor cells. It plays a critical role in regulating the activity of G protein-coupled receptors (GPCRs), particularly rhodopsin, the light-sensitive receptor in rod cells. Following light activation, GRK1 phosphorylates the cytoplasmic tail of rhodopsin, initiating its desensitization by promoting arrestin binding. This process is essential for terminating phototransduction signals and enabling photoreceptor recovery, ensuring proper visual function. Structurally, GRK1 contains a conserved kinase domain and a regulator of G protein signaling (RGS) domain, which facilitates interactions with activated G proteins.
Antibodies targeting GRK1 are vital tools for studying retinal biology and GPCR signaling mechanisms. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect GRK1 expression, localization, and post-translational modifications in retinal tissues or cell models. Dysregulation of GRK1 has been linked to retinal disorders such as Oguchi disease, a congenital stationary night blindness caused by GRK1 mutations. Research using GRK1 antibodies also explores its interaction with other proteins, such as recoverin, which modulates its kinase activity in a calcium-dependent manner. These studies contribute to understanding vision-related pathologies and potential therapeutic strategies, including gene therapy for inherited retinal diseases. Commercial GRK1 antibodies are typically raised in rabbits or mice, with validation in knockout controls to ensure specificity.
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