| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HMG2 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HMGB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HMGB2抗体的3篇虚构参考文献(基于常见研究方向整理，非真实文献)：
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1. **文献名称**：*HMGB2 promotes colorectal cancer metastasis via TLR4-mediated inflammatory signaling*
**作者**：Chen L, Wang Y, et al.
**摘要**：本研究利用HMGB2特异性抗体通过免疫组化(IHC)和Western blot技术，发现HMGB2在结直肠癌组织中高表达，并通过激活TLR4/NF-κB通路增强肿瘤细胞的侵袭和转移能力。
2. **文献名称**：*HMGB2 maintains stemness of embryonic stem cells through transcriptional regulation of pluripotency factors*
**作者**：Tanaka R, Sato H.
**摘要**：通过HMGB2抗体的染色质免疫沉淀(ChIP)实验，揭示HMGB2直接结合OCT4和NANOG基因启动子区域，调控胚胎干细胞的自我更新能力，敲低HMGB2导致干细胞分化。
3. **文献名称**：*Development of a high-specificity monoclonal antibody for HMGB2 and its application in glioblastoma diagnosis*
**作者**：Zhang Q, Liu F, et al.
**摘要**：报道一种新型HMGB2单克隆抗体的制备与验证，该抗体在ELISA和免疫荧光中表现出高特异性，并证实HMGB2在胶质母细胞瘤患者血清中显著升高，提示其作为诊断标志物的潜力。
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注：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science检索真实发表的论文。
**Background of HMGB2 Antibody**
The High Mobility Group Box 2 (HMGB2) protein is a non-histone chromatin-associated protein belonging to the HMGB family, which plays critical roles in DNA repair, recombination, transcription regulation, and chromatin remodeling. HMGB2 is highly expressed in embryonic tissues and certain cancer cells, where it contributes to cell proliferation, differentiation, and survival. Its function is closely linked to its ability to bind DNA and modulate chromatin structure, facilitating interactions between transcription factors and DNA.
HMGB2-specific antibodies are essential tools for studying its expression, localization, and interactions in cellular processes. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect HMGB2 in tissue samples, cultured cells, or experimental models. Research has highlighted HMGB2's involvement in cancer progression, stem cell biology, and inflammatory responses, with studies suggesting its potential as a biomarker or therapeutic target.
Antibodies targeting HMGB2 are typically validated for specificity against its conserved domains, distinguishing it from homologous family members like HMGB1. Their applications extend to investigating HMGB2's extracellular roles, where it may act as a damage-associated molecular pattern (DAMP) molecule, promoting inflammation or immune activation. Understanding HMGB2 dynamics through antibody-based assays continues to advance insights into developmental biology, oncogenesis, and regenerative medicine.
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