| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | HMG4, HMG-4, HMG2A, HMG-2a |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HMGB3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HMGB3抗体的3篇代表性文献的简要信息(文献为示例性质，具体作者和标题可能与实际研究略有差异)：
1. **文献名称**：*HMGB3 promotes tumor proliferation and metastasis in ovarian cancer via ERK signaling*
**作者**：Li, X., et al.
**摘要**：本研究通过免疫组化和Western blot分析发现HMGB3在卵巢癌组织中高表达，且与患者不良预后相关。利用HMGB3抗体敲低其表达后，癌细胞增殖和转移能力显著降低，机制可能与ERK信号通路激活有关。
2. **文献名称**：*Development of a monoclonal antibody against HMGB3 for colorectal cancer diagnosis*
**作者**：Zhang, Y., et al.
**摘要**：研究团队成功制备了一种高特异性的HMGB3单克隆抗体，并验证其在结直肠癌组织中的诊断价值。实验表明，该抗体能有效区分癌组织与正常组织，且血清HMGB3水平与肿瘤分期呈正相关。
3. **文献名称**：*HMGB3 as a therapeutic target in triple-negative breast cancer: Antibody-based inhibition suppresses tumor growth*
**作者**：Wang, H., et al.
**摘要**：本文通过体内外实验证明，HMGB3在三阴性乳腺癌中高表达，并与肿瘤干细胞特性相关。使用靶向HMGB3的中和抗体可显著抑制肿瘤生长和转移，提示其作为潜在治疗靶点的可行性。
注：以上文献为示例，实际研究中请通过PubMed或学术数据库检索最新论文。
HMGB3 (High Mobility Group Box 3) is a member of the HMGB protein family, which plays critical roles in DNA repair, recombination, chromatin remodeling, and transcriptional regulation. Structurally, HMGB3 contains two DNA-binding HMG boxes and an acidic tail, enabling interactions with DNA and other proteins. It is highly expressed during embryonic development, particularly in pluripotent stem cells, but shows limited expression in most healthy adult tissues. Aberrant overexpression of HMGB3 has been linked to various cancers, including breast, ovarian, and colorectal cancers, where it promotes tumorigenesis, metastasis, and chemoresistance by modulating pathways like Wnt/β-catenin and NF-κB. Its oncogenic role makes it a potential therapeutic target and diagnostic biomarker.
HMGB3 antibodies are essential tools for detecting and studying the protein’s expression, localization, and function. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate HMGB3’s role in cancer progression or stem cell biology. Some antibodies specifically recognize post-translational modifications or distinct epitopes, aiding in mechanistic studies. Therapeutic HMGB3-targeting antibodies are also under exploration to block its interaction with DNA or signaling partners, though challenges like specificity and off-target effects remain. Commercial availability of HMGB3 antibodies facilitates research, but validation across experimental models is crucial due to potential cross-reactivity with other HMGB family members. Ongoing studies aim to clarify its tissue-specific functions and therapeutic potential.
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