首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠睫状神经营养因子(CNTF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的CNTF含量。CNTF即睫状神经营养因子，是神经营养因子家族成员。它能维持多种神经元的存活和表型。研究机制上，CNTF经特定受体信号通路，激活相关蛋白和基因表达，在神经损伤修复、神经保护等方面研究较多。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于神经退行性疾病模型研究、药物干预后CNTF表达水平追踪、体外细胞实验中神经营养因子分泌动态监测等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CntfCiliary neurotrophic factor ELISA kit; CNTF ELISA kit
缩写：CNTF
Uniprot No.：P51642
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CNTF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 87-94 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of mouse CNTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 99 | 95-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4003.0182.9222.9702.8482002.3902.3842.3872.2651001.5281.5651.5471.425500.9200.9240.9220.800250.5730.6200.5970.47512.50.3720.3810.3770.2556.250.2560.2420.2490.12700.1220.1210.122 | ||||||
货期：3-5 working days
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