首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠C肽(C-Peptide)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C-Peptide含量。C-Peptide即C肽，是胰岛素原裂解产生的等分子肽类片段。它可反映内源性胰岛素分泌情况。研究机制上，C肽具有改善神经传导、增加肾血流量等作用，其与糖尿病并发症、肾脏疾病等的关系是研究热点。试剂盒检测范围为1.25 ng/mL-20 ng/mL，作为评估胰岛β细胞功能的重要生物标志物，广泛应用于糖尿病发病机制、胰岛功能调控及相关药物干预研究领域。适用于评估实验动物模型中的胰岛素分泌动态、胰腺组织功能状态及代谢相关研究。为糖尿病研究、药物作用评价及代谢调控机制探索提供可靠的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C-Peptide
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-20 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat C-peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 93-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat C-peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 96 | 91-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.1550.1510.153 100.2890.3040.297 50.5010.4910.496 2.50.8470.9120.880 1.251.9541.8261.890 | ||||||
货期：3-5 working days
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