| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKAA1 |
| Uniprot No | Q13131 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-279aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMRRLSSWRKMATAEKQKHDGRVKIGHY ILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQ NLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKES RRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSD GEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDD DHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHE WF |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRKAA1重组蛋白的3篇代表性文献的模拟示例(基于领域内常见研究方向，非真实文献)：
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1. **文献名称**：*Expression and purification of active recombinant human PRKAA1 in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：本研究描述了一种在大肠杆菌中高效表达和纯化功能性人源PRKAA1重组蛋白的方法。通过优化密码子使用和诱导条件，获得了可溶性蛋白，并利用亲和层析和尺寸排阻色谱纯化，验证了其AMP依赖的激酶活性。
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2. **文献名称**：*Structural insights into the autoinhibition and AMPK activation mechanism of PRKAA1*
**作者**：Li Y, et al.
**摘要**：该研究通过重组表达PRKAA1及其调控亚基，解析了其自抑制状态和AMP激活下的晶体结构，揭示了ATP结合域构象变化在激酶活性调控中的关键作用。
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3. **文献名称**：*Development of a high-throughput screening assay for PRKAA1 activators using recombinant protein*
**作者**：Wang H, et al.
**摘要**：利用重组PRKAA1蛋白构建了一种基于荧光共振能量转移(FRET)的高通量筛选平台，用于鉴定新型AMPK激活剂，为代谢性疾病药物开发提供工具。
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**备注**：以上文献为模拟示例，实际引用时请通过PubMed或Google Scholar搜索真实文献(关键词：PRKAA1 recombinant, AMPK alpha1 purification)。
PRKAA1 (Protein Kinase AMP-Activated Catalytic Subunit Alpha 1), also known as AMPKα1. is a critical component of the AMP-activated protein kinase (AMPK) complex, a central regulator of cellular energy homeostasis. This serine/threonine kinase functions as a metabolic sensor, activated under low-energy conditions (e.g., ATP depletion, hypoxia, or nutrient stress) to restore energy balance by inhibiting anabolic pathways and promoting catabolic processes. The PRKAA1 gene encodes the α1 catalytic subunit, which contains a kinase domain responsible for phosphorylating downstream targets, along with regulatory regions that interact with β and γ subunits to form the functional heterotrimeric AMPK complex.
Recombinant PRKAA1 protein is engineered using expression systems such as *E. coli*, insect cells, or mammalian cells to produce purified, bioactive forms of the enzyme for research and therapeutic development. Its production often involves tagging (e.g., GST, His-tag) to facilitate purification and detection. Researchers utilize recombinant PRKAA1 to study AMPK signaling mechanisms, including its activation by upstream kinases (e.g., LKB1. CaMKKβ) and allosteric modulators like AMP/ADP. It is also employed in high-throughput drug screens to identify AMPK activators or inhibitors, which hold therapeutic potential for metabolic disorders (e.g., type 2 diabetes, obesity), cancer, and neurodegenerative diseases.
Structural studies using recombinant PRKAA1 have elucidated conformational changes during activation and its interaction with small molecules, aiding rational drug design. Additionally, it serves as a tool to investigate tissue-specific roles of AMPKα1 isoforms, particularly in the liver, skeletal muscle, and brain, where it regulates glucose uptake, lipid metabolism, and mitochondrial biogenesis. Dysregulation of PRKAA1 is linked to insulin resistance, tumor progression, and cardiovascular diseases, underscoring its biomedical relevance.
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