首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠巨噬细胞集落刺激因子1受体(CSF1R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CSF1R含量。CSF1R，即集落刺激因子1受体，是一种酪氨酸激酶跨膜受体，属于生长因子CSF-1/血小板衍生生长因子（PDGF）受体家族。它参与调节单核吞噬细胞（如巨噬细胞、单核细胞）的存活、增殖、分化、招募和功能。CSF1R的研究机制涉及其在肿瘤微环境中的作用，以及与IL-34和CSF-1的相互作用，对肿瘤免疫疗效有重要影响。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外研究巨噬细胞功能调控、免疫应答机制探索及相关疾病模型的分子机制分析。该产品严格进行批次间质控，提供详细的实验方案和标准曲线参考，为科研人员在神经退行性疾病、肿瘤免疫或组织修复等领域的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Csf1r ELISA kit; Csfmr ELISA kit; FmsMacrophage colony-stimulating factor 1 receptor ELISA kit; CSF-1 receptor ELISA kit; CSF-1-R ELISA kit; CSF-1R ELISA kit; M-CSF-R ELISA kit; EC 2.7.10.1 ELISA kit; Proto-oncogene c-Fms ELISA kit; CD antigen CD115 ELISA kit
缩写：CSF1R
Uniprot No.：Q00495
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CSF1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 86-92 | ||||||
| 1:2 | Average % | 108 | |||||
| Range % | 104-112 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
回收率：
| The recovery of rat CSF1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 80-88 | |||||
| EDTA plasma (n=4) | 107 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.4662.4382.4522.349101.8671.9021.8851.78251.2391.2681.2541.1512.50.7510.7380.7450.6421.250.4450.4510.4480.3450.6250.2870.2690.2780.1750.3120.2070.2100.2090.10600.1020.1040.103 | |||||||
货期：3-5 working days
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