首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠粒细胞巨噬细胞集落刺激因子(GM-CSF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CSF2含量。CSF2即集落刺激因子2，主要作用是刺激造血干细胞生长、分化和成熟，在免疫调节中发挥重要作用。研究机制上，其通过与受体结合激活下游信号通路，调控细胞的增殖和存活，在肿瘤治疗、免疫相关疾病研究等方面有较大潜在价值。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。广泛应用于免疫机制研究、疾病动物模型评估及药物开发中的GM-CSF动态监测，例如败血症模型中细胞因子风暴分析、肿瘤免疫治疗药物对髓系细胞的影响评估等科研场景，特别适用于微量样本的高通量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Csf2 ELISA Kit; Csfgm ELISA Kit; Granulocyte-macrophage colony-stimulating factor ELISA Kit; GM-CSF ELISA Kit; Colony-stimulating factor ELISA Kit; CSF ELISA Kit
缩写：CSF2
Uniprot No.：P01587
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-100 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 84-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of mouse GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 83-103 | ||||
| EDTA plasma (n=4) | 96 | 89-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7622.8562.8092.6335002.1902.2232.2072.0312501.4111.4351.4231.2471250.8450.8560.8510.67562.50.5560.5610.5590.38331.20.3780.3820.3800.20415.60.2540.2690.2620.08600.1750.1760.176 | ||||||
货期：3-5 working days
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