| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/3000-1/10000 | Human,Mouse,Rat |
| Aliases | TP1; TLP1; p240; TROVE1; VAULT2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TEP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TEP1抗体的3篇参考文献，涵盖不同研究方向：
1. **文献名称**：*Complement-like protein TEP1 is a determinant of vectorial capacity in malaria mosquitoes*
**作者**：Blandin, S., et al.
**摘要**：该研究通过使用抗TEP1抗体中和按蚊体内的TEP1蛋白，证明其是疟原虫在蚊体内发育的关键免疫因子。抗体阻断实验显示，TEP1缺失显著增加疟原虫卵囊存活率，表明其在抗疟免疫中的直接作用。
2. **文献名称**：*Conserved role of a complement-like protein in phagocytosis revealed by dsRNA knockout in cultured cells of the mosquito, Anopheles gambiae*
**作者**：Levashina, E.A., et al.
**摘要**：研究利用RNA干扰和抗TEP1抗体，证明按蚊TEP1通过调理作用促进病原体的吞噬清除。抗体介导的功能抑制实验揭示了TEP1在先天免疫中的保守机制。
3. **文献名称**：*Telomerase-associated protein TEP1: Not essential for telomerase activity but required for processivity and telomere length maintenance*
**作者**：Xin, H., et al.
**摘要**：该文献通过免疫沉淀(使用TEP1特异性抗体)发现，TEP1虽不直接参与端粒酶催化活性，但影响其延伸端粒的持续性和长度调控，揭示了其在端粒酶复合体中的结构功能。
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**备注**：TEP1在不同领域含义不同：
- **按蚊免疫**：指Thioester-containing protein 1.属补体样蛋白。
- **端粒酶相关**：指Telomerase-associated protein 1.参与端粒维持。
上述文献分别覆盖两类研究方向，需根据具体应用场景选择。
The Telomerase-associated protein 1 (TEP1), also known as p230. is a core component of the telomerase complex, an enzyme critical for maintaining telomere integrity by adding repetitive nucleotide sequences to chromosome ends. TEP1 interacts with the telomerase RNA component (TERC) and the catalytic subunit (TERT), stabilizing the complex and facilitating its nuclear localization. Initially identified in *Tetrahymena* and later conserved in vertebrates, TEP1 contains multiple protein interaction domains, including a dyskerin-like domain, suggesting roles in RNA binding and complex assembly.
Antibodies targeting TEP1 are essential tools for studying telomerase biology, particularly in cancer research, as telomerase reactivation is a hallmark of ~90% of human cancers. These antibodies enable detection of TEP1 expression via techniques like Western blotting, immunohistochemistry, and immunoprecipitation, aiding in investigations of telomerase regulation, cellular aging, and oncogenesis. However, challenges persist due to TEP1's structural homology with other proteins and low abundance in somatic cells, requiring stringent validation of antibody specificity. Recent studies also explore TEP1's non-canonical roles in processes like DNA repair and stem cell maintenance, expanding its therapeutic relevance. Commercial TEP1 antibodies are typically raised against conserved epitopes in human or murine models, supporting both basic research and drug development efforts targeting telomerase-related pathologies.
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