首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠β连环蛋白/联蛋白(β-Cat)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTNNB1含量。CTNNB1（β-catenin）是一种参与细胞粘附和Wnt信号通路的蛋白，其突变与多种癌症相关。研究显示，CTNNB1突变可稳定β-catenin蛋白，导致其移位至细胞核并激活Wnt信号通路，进而促进肿瘤生长。目前，针对CTNNB1的靶向治疗研究正在积极进行中。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于研究β-Cat在肿瘤模型、干细胞调控或胚胎发育等领域的表达动态，为探索Wnt信号通路机制提供高效工具。试剂盒支持多种样本类型检测，可满足体外实验、动物模型等科研场景需求，但仅限科学研究使用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctnnb1 ELISA Kit; CatnbCatenin beta-1 ELISA Kit; Beta-catenin ELISA Kit
缩写：CTNNB1
Uniprot No.：Q02248
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-cat in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 94-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of mouse β-cat spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-98 | ||||
| EDTA plasma (n=4) | 99 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.8542.9612.9082.71552.1562.2442.2002.0072.51.4331.4981.4661.2731.250.7740.7680.7710.5780.6250.4570.4430.4500.2570.3120.3240.3390.3320.1390.1560.2450.2280.2370.04400.1890.1970.193 | ||||||
货期：3-5 working days
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