首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠干扰素诱导蛋白10(IP-10/CXCL10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL10含量。CXCL10是一个重要的靶点。它属于趋化因子家族，在免疫调节等生理过程中发挥关键作用。其背景涉及多种疾病，如炎症、肿瘤等。研究机制方面，主要聚焦于它与受体结合，介导细胞趋化、激活免疫细胞等，以揭示其在疾病发生发展中的具体作用。试剂盒检测范围为781.25 pg/mL-50000 pg/mL，适用于基础科研中评估炎症模型（如肝炎、脑炎或关节炎）、免疫调节机制研究、肿瘤相关免疫微环境分析，以及药物干预或基因修饰对IP-10/CXCL10表达调控的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cxcl10 ELISA kit; Inp10 ELISA kit; Mob1 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; Interferon-inducible protein 10 ELISA kit; Protein Mob-1 ELISA kit; Small-inducible cytokine B10 ELISA kit
缩写：CXCL10
Uniprot No.：P48973
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：781.25 pg/mL-50000 pg/mL
灵敏度：195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of rat IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 93-96 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected500002.5562.5812.5692.437250001.7481.7691.7591.627125001.0021.0851.0440.91262500.6630.6990.6810.54931250.3960.3870.3920.2601562.50.2840.2710.2780.146781.250.2190.2300.2250.09300.1310.1330.132 | ||||||
货期：3-5 working days
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