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Mouse Macrophage Inflammatory Protein 2,MIP-2 ELISA kit

  • 中文名: 小鼠巨噬细胞炎性蛋白2(MIP-2)ELISA Kit
货号: IPD-E04666m
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠巨噬细胞炎性蛋白2(MIP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CXCL2含量。CXCL2为趋化因子,可趋化中性粒细胞迁移。其在多种病理如炎症、肿瘤等过程中表达异常。研究机制上,它主要通过结合相应受体,激活下游信号通路,参与细胞的增殖、迁移和免疫调节等,成为疾病治疗潜在靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL,灵敏度为5.8 pg/mL。本试剂盒适用于科研场景中炎症机制研究、药物干预效果评价或疾病动物模型分析,为探索MIP - 2在炎症相关通路中的作用提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Cxcl2 ELISA Kit; Mip-2 ELISA Kit; Mip2 ELISA Kit; Scyb2C-X-C motif chemokine 2 ELISA Kit; Macrophage inflammatory protein 2 ELISA Kit; MIP2 ELISA Kit
  • 缩写:
    CXCL2
  • Uniprot No.:
    P10889
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    23.5 pg/mL-1500 pg/mL
  • 灵敏度:
    5.8 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    83-94


    1:2

    Average %

    95


    Range %

    90-102


    1:4

    Average %

    94


    Range %

    92-97


    1:8

    Average %

    93


    Range %

    89-98


  • 回收率:

    The recovery of mouse MIP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    88-95


    EDTA plasma (n=4)

    93

    88-100


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1500

    3.069

    2.934

    3.001

    2.852


    750

    2.099

    1.967

    2.033

    1.884


    375

    1.191

    1.128

    1.160

    1.010


    187.5

    0.654

    0.629

    0.642

    0.493


    94

    0.387

    0.391

    0.389

    0.240


    47

    0.273

    0.253

    0.263

    0.114


    23.5

    0.192

    0.184

    0.188

    0.039


    0

    0.153

    0.145

    0.149




  • 货期:
    3-5 working days

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