| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NSE1; FAM84A; PP11517 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human LRATD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是假设存在的关于NEK5抗体的参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：*NEK5 Kinase Regulates Breast Cancer Cell Migration through Focal Adhesion Dynamics*
**作者**：Smith A, et al.
**摘要**：本研究利用特异性NEK5抗体，发现NEK5通过磷酸化黏着斑蛋白(如Paxillin)调控乳腺癌细胞迁移。Western blot和免疫荧光显示NEK5在侵袭性乳腺癌细胞中高表达，其抑制可减少细胞侵袭能力。
2. **文献名称**：*Development and Validation of a Polyclonal Antibody for NEK5 Detection in Human Tissues*
**作者**：Li H, et al.
**摘要**：文章报道了一种兔源多克隆NEK5抗体的制备与验证。该抗体在免疫组化(IHC)和免疫沉淀(IP)中表现出高特异性，成功应用于前列腺癌组织样本中NEK5蛋白水平的定量分析。
3. **文献名称**：*NEK5-Mediated DNA Damage Response in Glioblastoma Stem Cells*
**作者**：Park J, et al.
**摘要**：通过NEK5抗体阻断实验，发现NEK5在胶质母细胞瘤干细胞中通过激活ATM/ATR通路参与DNA损伤修复。研究提示NEK5可能成为靶向治疗耐药性肿瘤的潜在靶点。
4. **文献名称**：*Functional Characterization of NEK5 in Cardiac Hypertrophy*
**作者**：García-Rojas M, et al.
**摘要**：利用NEK5特异性抗体发现，NEK5在小鼠心肌肥厚模型中表达上调，并通过调控NF-κB信号通路促进病理性心脏重塑。抗体敲低实验证实其功能必要性。
(注：以上为模拟内容，实际文献需通过学术数据库检索确认。)
**Background of NEK5 Antibody**
NEK5 (NIMA-related kinase 5) belongs to the NEK family of serine/threonine kinases, which are implicated in cell cycle regulation, particularly in mitotic progression and DNA damage response. While other NEK family members, such as NEK2 and NEK6. are well-studied, NEK5 remains less characterized. Current evidence suggests NEK5 may play roles in centrosome maturation, cilia formation, and cellular stress responses. Its dysregulation has been tentatively linked to cancers, including breast and prostate cancer, though mechanistic insights remain limited.
NEK5 antibodies are critical tools for detecting and studying the expression, localization, and function of NEK5 in biological systems. These antibodies enable techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to explore NEK5's role in health and disease. Due to the kinase's low abundance and sequence homology with other NEKs, high specificity is essential for reliable results. Commercial NEK5 antibodies are typically validated for cross-reactivity and affinity to ensure accurate detection.
Research using NEK5 antibodies has begun uncovering its potential as a therapeutic target, particularly in oncology. However, gaps persist in understanding its substrates, signaling pathways, and regulatory mechanisms. Ongoing studies aim to clarify NEK5's contributions to cellular processes and pathologies, highlighting the antibody's importance in advancing molecular and clinical research.
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