| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | AAT6; ACTSA; MYMY5 |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ACTA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ACTA2抗体的3篇代表性文献(均为虚构示例，仅作格式参考)：
1. **"ACTA2 as a marker of vascular smooth muscle differentiation in atherosclerosis"**
- **作者**: Smith J, et al.
- **摘要**: 本研究验证了ACTA2抗体在动脉粥样硬化模型中特异性标记血管平滑肌细胞(VSMCs)的能力，并发现其表达水平与疾病进展相关。
2. **"Role of ACTA2-positive fibroblasts in tumor stroma remodeling"**
- **作者**: Lee H, et al.
- **摘要**: 通过免疫组化(ACTA2抗体)和单细胞测序，揭示了肿瘤微环境中ACTA2+成纤维细胞通过胶原沉积促进癌症侵袭的机制。
3. **"ACTA2 antibody specificity in fibrosis: Comparison across species"**
- **作者**: Brown K, et al.
- **摘要**: 评估了不同物种(人、小鼠、大鼠)组织中ACTA2抗体的交叉反应性，确认其作为肌成纤维细胞活化标志物在肝纤维化模型中的可靠性。
如需真实文献，建议通过PubMed搜索关键词“ACTA2 antibody”或“α-SMA antibody”筛选应用类研究。
The ACTA2 antibody targets alpha-smooth muscle actin (α-SMA), a protein encoded by the ACTA2 gene. This cytoskeletal protein is predominantly expressed in smooth muscle cells, where it plays a critical role in cell contraction, motility, and structural integrity. α-SMA is a hallmark of vascular smooth muscle cells, myofibroblasts, and pericytes, making it a key marker for studying tissue remodeling, fibrosis, and vascular pathologies.
In research, ACTA2 antibodies are widely used to identify smooth muscle cell populations in tissues, assess myofibroblast activation during wound healing or fibrosis, and investigate tumor stroma in cancer biology. They are essential tools in techniques like immunohistochemistry, Western blotting, and flow cytometry. Pathologically, elevated α-SMA expression is linked to fibrotic disorders (e.g., liver cirrhosis, pulmonary fibrosis) and vascular diseases (e.g., atherosclerosis, aneurysms). Conversely, ACTA2 gene mutations are associated with hereditary thoracic aortic diseases, driving studies on vascular smooth muscle dysfunction.
Commercial ACTA2 antibodies are typically raised against conserved epitopes, ensuring reactivity across human, mouse, and rat samples. Validation includes specificity checks in α-SMA-rich tissues (e.g., blood vessels) and negative controls in non-muscle cells. Researchers also utilize these antibodies to explore cellular responses to mechanical stress, hypoxia, or TGF-β signaling, reflecting α-SMA's role in adaptive tissue responses. Their application spans basic research, diagnostic pathology, and therapeutic target validation.
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